豆野螟触角化感器和性信息素分泌腺的位置及超微结构

Location and ultrastructure observation on the antennal sensilla and sex pheromone gland in Maruca testulalis Geyer

利用扫锚电镜技术研究了豆野螟Maruca testulalis Geyer成虫触角感器和性信息素腺体的位置及超微结构,结果表明：豆野螟雌雄蛾的触角呈线状,由柄节、梗节和鞭节组成,柄节较长、梗节较粗,并均覆盖着鳞片;触角鞭节由72-82节组成,长度约为86-97 mm;豆野螟感受器主要分布在触角的腹面和侧面,背面覆盖有鳞片,因此感受器很少;雌雄触角共发现6种感器,其中柱形感器只在雄蛾中发现,而一种未定义的感器仅在雌蛾中发现。通过形态比较发现,毛形感器在雌蛾触角上的分布比雄蛾较稀疏,但雄蛾的长度略长,其他几种感器在雌雄间未见显著差异;豆野螟性信息素腺体位于腹部第8-9节之间,其上有一系列规则的皱褶,为性腺细胞所在位置。使用气质联用技术进一步对豆野螟性信息素腺体进行了定位,测定结果表明,腹部第8-9节间的腺体提取物具有性信息素组分,是豆野螟性腺所在位置。

The morphology of antennae sensilla and sex pheromone gland of Maruca testulalis Geyer were observed under scanning electron microscope.Antennae were made up of scapus,pedicle and flagella.The latter consisted of 72-82 segments and was about 86-97 cm long.The upper surface of antennae was covered with cataphylla and most of the antennal sensilla lied on its side and lower surfaces.Six kinds of antennal sensilla were found in both sexes.Sensilla cylindric were observed on male antennae and an undefined sensilla was observed on female antennae only.Sensilla trichodeum of males were longer than that of females.The number of sensillum trichodeum was lesser on females antennae than males antennae.The study showed the sex pheromone gland was situated in the intersegmental membrane between the eighth and ninth abdominal segments,which was verified by GC-MS analysis.