摘要
目的采用热水、乙醇、盐酸提取面包酵母中的还原型谷胱甘肽,获得最优的提取方法。方法在单因素试验的基础上对乙醇提取法的乙醇浓度、pH、温度、提取时间进行L9(34)正交试验并采用二次提取验证提取效果。结果在40℃,pH 1.0,乙醇含量50%条件下提取40 min,能有效地提取还原型谷胱甘肽,采用二次提取法验证其提取率达到95.4%。结论与其它方法相比,乙醇提取法是一种简便、快速、经济、有效的方法。
Objective To extract glutathione（GSH） from baker＇s yeast with hot water,ethanol,acid respectively to get the best extraction method.Methods Based on the single factor experiment,the optimal conditions for ethanol extraction were obtained by L9（34） orthogonal design and were validated by secondary extraction.Results GSH was effectively extracted with 50% ethanol for 40 min at 40 ℃and pH 1.0.The extraction rate reached 95.4%,validated by secondary extraction method.Conclusion Compared with other methods,ethanol extraction method proved to be a convenient,fast and effective method for the extraction of glutathione.
出处
《食品与药品》
CAS
2011年第7期234-237,共4页
Food and Drug
