5种商品化解脲脲原体培养基质量评价研究

Evaluation research of five kinds of commercially available culture media for detecting ureaplasma urealyticum

目的评价商品化解脲脲原体(Uu)培养基的质量。方法应用标准菌株Uu4和Uu14比较5种培养基(M、K、L、H、A)的敏感性;以固体培养基的结果作为金标准,应用Uu临床菌株比较5种培养基的敏感性、特异性、阳性预测值、阴性预测值及和金标准的一致度。结果 M和K培养基敏感性最高;M、K、L和H培养基的敏感性>90.00%;M、K、A培养基特异性达到100.00%;M、K、A阳性预测值为100.00%,而M、K阴性预测值最高,均为99.24%;M、K、L和H培养基与固体培养基一致度满意,而A培养基一致度仅相对满意。结论 M、K培养基质量最佳。市场上检测Uu的培养基存在质量差异,临床上选用宜慎重。

Objective To evaluate the quality of commercially available culture medium for detecting ureaplasmau realyticum（Uu）.Methods The sensitivities of 5 culture media（M,K,L,H and A） with standard strains Uu4 andUu14 were compared,and the comparison result of solid culture media was performed as the gold standard.The sensitivity,specificity,positive predictive value and negative predictive value of the 5 culture media with the gold standard were compared by clinical bacterium Uu so as to determine the consistency.Results According to the comparison results,the sensitivities of culture media M and K were the highest.The sensitivities of culture media M,K,L and H were 90.00%,while the specificities of culture media M,K and A were 100.00%.The positive predictivevalues of culture media M,K and A were 100.00%.The negative predictive values of culture media M and K（99.24%） were the highest.The comparison of culture medium M,K,L and H with the solid culture media had a satisfactory consistency,while culture medium A had a comparative satisfactory consistency.Conclusions The qualities of culture media M and K are good.Since the culture media designed for Uu detection in the market are largely different in quality,the Uu culture media should be re-evaluated before being applied for clinical diagnosis.