大鼠急性放射性肝损伤模型的建立

Establishment model of acute radiation Induced liver injury of rats

目的：建立大鼠急性放射性肝损伤模型．方法：雌性SD大鼠10只为正常阴性对照组，12只为照射组。对照组为正常饲养4周；照射组大鼠肝脏右叶均接受6兆伏（MV）-X线源皮照射技术,源皮距离（SSD）为80cm，照射深度为2cm，照射速度（吸收剂量率）为300cGy／min，单次照射总量为20Gy。每周观察大鼠的一般生活状态；4周后大鼠眼内眦静脉取血，检测血清谷丙转氨酶（ALT）、血清谷草转氨酶（AST）水平；普通光学显微镜下观察肝脏组织的病理形态学变化。结果：大鼠肝右叶接受单次辐射20Gy后2周，其一般生活状态差，II放射性皮炎，4用后大鼠存活率为91．7％（11／12）；显微镜下大鼠肝脏右叶发生不同程度的损伤，主要表现为汇管区炎性细胞浸润、肝血窭水肿；照射组大鼠血清ALT为（44．00±11．95）U／L，高于对照组（26．00±9．63）U／L，t=2．8725，P=0．0166；照射组大鼠血AST为（108．50±20．69）U／L，高于对照组（69．17±8．84）U／U，t=4．2828，P=0．0016，P值均〈0．05，差异均具有统计学意义。结论：成功建立大鼠急性放射性肝损伤模型。

Objectlve： To establish acute radiation induced liver injury of rats. Methods： 10 female SD rats as normal negative control group and 12 female SD rats as irradiation group who were accepted 20Gy 6mv-X ray on the right lobe of the liver. Source skin distance（SSD） is 80em, the depth of irra- diation is 2cm, absorbed dose rate is 300cGy/min. The rats were bred in the same condition for 4 weeks and observed the following index.-a, the general conditions, b. Checking the ALT, AST level in serum, c. By the H--E staining, observing the pathomorphologieal change of liver. Results： Those rats that accepted 20Gy irradiation were in a poor general condition with II radiation dermatitis two weeks after irradition. Survival rate was 91.7%（11/12） in four weeks. In irradiation group, there are some injury of right lobe liver, such as edema of hepatic sinusoid and inflammation of hepatic header. Irradia- tion group＇s ALT （44.00±11.95U/L） and AST（108.50±20.69U/L was higher than that of in control group＇s （26.00＋9.63U/L） and （69.17±8.84U/ L）, respectively. Conclusions： Modeling of irradiation induced liver injury of rats were successful.