红藤诱发胃腺癌细胞MCGS03凋亡的研究

The study on apoptosis and cell cycle arrest of MCGS03 cells induced by caulis sargentodoxae prescription

目的：观察红藤对胃腺癌细胞MCG803细胞生长周期分布及细胞凋亡的影响。方法；采用MTT比色法观察10，20，40，80和160mg／L红藤处理48h对胃腺癌细胞MCG803细胞生长的影响，采用Gimesa染色技术、荧光染色技术进行MCGS03细胞凋亡检测，并用流式细胞仪检测40mg／L红藤处理24h对MCG803细胞周期分布的影响。结果：红藤可抑制胃腺癌细胞MCG803的增殖，红藤与细胞培养12小时即有少数MCG803细胞发生凋亡，随时间延长凋亡细胞数量逐渐增多，改变细胞周期分布，使G-0／G1期细胞比例增高，S期比例降低，并对MCG803细胞有促凋亡作用。结论：红藤可改变细胞周期分布，影响细胞周期调控蛋白表达，并可诱导MCG803细胞凋亡，从而抑制细胞增殖。

Objective： To study the effects of caulis sargentodoxae prescription（CSP） on cell proliferation and apoptosis on MCGS03 cells and to eluci- date its molecular mechanism. Methods： The proportion of the periodic tumor cells were altered by 10, 20, 40,80, 160 mg/L CSP treated 48 h using MTT and Sub--G1 curves were displayed at 40 mg/L CSP treated 24h by flow cytometry analysis. With Giemsa staining and fluorescence staining assay to detect the MCGS03 apoptosis. Results： CSP significantly Suppressed MCGS03 cell proliferation in a time-- and dose--dependent manner. The proportion of the periodic tumor ceils were altered by CSPo CSP decreased the proportion of cells in S phase and increased the proportion of cells in G0/G1 and/or G2/M phases of the cell cycle. Conclusion： These data support that CSP can induce apoptosis and alert cell cycle arrest of the Human Gliomaous Cells MCGS03