摘要
目的:研究复方三芪提取物对由N-甲基-D天门冬氨酸(NMDA)诱导的大鼠视网膜损伤模型的保护作用.方法:20只SD大鼠随机分为5组:正常对照组、模型组、复方三芪提取物低、中、高剂量组.模型组和给药组大鼠于右眼玻璃体内注射2μL 40 nmol.L-1NMDA,制备视网膜神经元损伤模型.给药组在NMDA注射前7 d起给予复方三芪提取物灌胃(3.903,7.805,15.61 g.kg-1.B.W.);正常对照组和模型组则给予等剂量的生理盐水灌胃.注射NMDA 7 d后静脉取血,测定血清超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量,取眼球组织,切片HE染色,观察视网膜厚度,并对视网膜视神经节细胞层(RGCL)神经元进行计数.结果:模型组大鼠RGCL神经元个数为正常组大鼠的51±6%.同模型组相比,复方三芪提取物(3.903,7.805,15.61 g.kg-1.B.W.)能剂量依赖性地增加RGCL神经元个数(P<0.05),依次为正常组的69±6%,89±7%,103±7%.复方三芪提取物(7.805 g.kg-1.B.W.)显著提高大鼠血清SOD活性并降低MDA含量(P<0.001).结论:复方三芪提取物能抑制NMDA诱导的视网膜脂质过氧化,并能阻止大鼠视网膜神经元进行性丢失,对NMDA诱导损伤的大鼠视网膜有修复作用.提示复方三芪提取物可能具有促进神经元的再生作用.
We investigated the neuroprotective effect of Compound Sanqi Extract(CSE) using a rat model of retinal damage induced by N-methyl-D-aspartate(NMDA) in the present study.Twenty Sprague-Dawley rats were divided into five groups: Control group,NMDA group and three CSE groups.The rats in NMDA group and CSE groups received intravitreal injection of NMDA(2 μL 40 nmol·L-1).Rats in CSE groups were orally given different dosages of CSE(3.903,7.805 and 15.61 g·kg-1.B.W.) seven days before NMDA treatment.Rats in the control and NMDA group received oral saline.Results showed that the percentage of neuron numbers in retinal ganglion cell layer(RGCL) greatly increased in a dose-dependent manner(69±6 %,89± 7 % and 103±7 % of control) after the seven days treatment with CSE as compared with NMDA group(51±6 % of control,P0.05).In addition,the treatment of rats with oral administration of 7.805 g·kg-1 CSE for seven days significantly increased superoxide dismutase(SOD) activity and decreased malondialdehyde(MDA) level in the serum as compared with NMDA group(P0.001).These findings indicated that CSE can reduce the NMDA-induced lipid peroxidation of rat retina by improving SOD activity and inhibiting MDA production,and which may stop the loss of retina ganglion cells and repair the damaged retina.
出处
《中南民族大学学报(自然科学版)》
CAS
2011年第1期54-58,共5页
Journal of South-Central University for Nationalities:Natural Science Edition
基金
国家自然科学基金资助项目(81070887)
中南民族大学基本科研业务费专项资金项目(CZQ10011)
中南民族大学科学研究基金(XTZ10001)