期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

nHA/PCL复合材料对成骨细胞增殖的影响 被引量：2

Effects of nHA/PCL Composites on the Osteoblast Proliferation
下载PDF
导出
摘要 研究了纳米羟基磷灰石/聚己内酯(nHA/PCL)复合材料对成骨细胞的增殖的影响.制备了3种不同的nHA/PCL材料浸提液(3种材料中nHA/PCL质量比分别为0∶100,20∶80和40∶60).采用四唑盐比色法(MTT)检测不同的nHA/PCL材料浸提液对成骨细胞增殖率的影响.应用流式细胞术检测复合材料对成骨细胞周期的影响.结果表明:与对照组相比,实验组细胞增殖率和细胞各时期细胞数量均无显著性差异(p>0.05);研究表明:nHA/PCL浸提液对成骨细胞的增殖无不良影响,具有良好的细胞的相容性,其中40%nHA/PCL具有促进成骨细胞增殖指数的作用. The effects of nano-hydroxyapatite/polycaprolactone（nHA/PCL） materials on osteoblast proliferation were investigated.Three different nHA/PCL materials′ extracts with mass rate of 0∶ 100,20∶ 80 and 40∶ 60 were prepared.MTT assay is used to determine the proliferation rate of osteoblast.Flow cytometry was applied to the effects of materials on the cell cycle of osteoblast,The results show that the cell cycle of osteoblasts in tested groups were not affected by the extract from control groups（p 0.05）.nHA/PCL composites used in this study have no negative effects on the osteoblast proliferation and their biocompatibility is shown.Among them,40 % nHA/PCL enhance PI of osteoblast.
作者 王朝元 李俊 刘介
机构地区 中南民族大学生命科学学院
出处 《中南民族大学学报（自然科学版）》 CAS 2011年第2期51-53,共3页 Journal of South-Central University for Nationalities：Natural Science Edition
基金 湖北省自然科学基金资助项目(2009CDZ033)
关键词 纳米羟基磷灰石/聚己内酯 成骨细胞 细胞增殖率 细胞周期 nano-hydroxyapatite/polycaprolactone（nHA/PCL） osteoblast cell proliferation cell cycle
分类号 Q813.1 [生物学—生物工程]
  • 相关文献

参考文献8

  • 1Aihemat Yusupu, Wang Zhenbin, Zhu Liang. Applica- tion of biodegradable polycaprolactone in medicine [ J]. Biomedical Engineering Foreign Medical Sciences, 2005, 28(1) : 19-22.
  • 2唐文胜,蒋电明.羟基磷灰石及其复合材料在骨修复中的作用及研究进展[J].中华创伤骨科杂志,2003,5(4):370-373. 被引量：17
  • 3Ylinen P. Filling of bone defeet swith porous hydroxyapa- tite reinforced with polylaetide or polyglyeolidefibres. Journal of Materials Seienee [ J ]. Materials in Medieine, 1994,5 (8) :522-528.
  • 4Causa F, Netti P A, Ambrosio L, eta;. Poly-epsiloncap- rolactone/hydroxyapatite composites for bone regenera- tion: in vitro characterization and human osteoblast re- sponse[ J ]. J Biomed Mater Res A, 2006,76:51-62.
  • 5Jayarama Reddy Venugopal, Sharon Low, Aw Tar Choon. Nanobioengineered "electrospun composite nanofibers and osteoblasts for bone regenera [ J ]. Artificial Organs, 2008,32(5) :388-397.
  • 6Ersev H, SehamLz G, Bayirli G, et al. Cytotoxic and mutagenic potencies of various root canal filling materials in eukaryotic and prokaryotic cells in vitro[ J]. J Endod, 1999,25 (5) :359-363 .
  • 7Sung K L, Frojmovic M M, O'Toole T E, et al. Determi- nation of adhesion force between single cell pairs genera- ted by activated GPIIB-III a receptors[ J]. Blood, 1993, 81(2) :419-423.
  • 8叶玲,苏勤,周学东.纳米羟磷灰石/聚酰胺66对成骨细胞生物学作用的实验研究[J].华西口腔医学杂志,2005,23(2):142-144. 被引量：6

二级参考文献34

  • 1马金忠.人工骨—羟基磷灰石[J].中华手外科杂志,1993,9(3):183-185. 被引量：2
  • 2Bilbe G, Roberts E, Birch M, etal. PCRphenotyping of cytokines in human osteoblast-like cell lines[J]. Bone, 1996,19(5) :437.
  • 3Buttery LD, Bourne S, Xynos JD, et al. Differentiation of osteoblast and in vitro bone formation from murine embryonic stem cell[J]. Tissue Eng, 2001,7(1):89-99.
  • 4Ersev H, Schmalz G, Bayirli G, et al. Cytotoxic and mutagenic potencies of various root canal filling materials in eukaryotic and prokaryotic cells in vitro[J]. J Endod, 1999,25(5):359-363.
  • 5Chelly J, Kaplan JC, Maire P, et al. Transcription of the dystrophin gene in human muscle and non-muscle tissue[J]. Nature, 1988,333(6176): 858-860.
  • 6Totoribe K, Tajima N, Chosa E, Matsumoto M, Kataoka H, Koono M. Hydroxyapatite block for use in posterolateral lumbar fusion. Clin Orthop, 2002, (399): 146-151.
  • 7Khodadadyan-Klostermann C, Liebig T, Melcher I, Raschke M,Haas NP. Osseous integration of hydroxyapatite grafts in metaphyseal bone defects of the proximal tibia (CT-study). Acta Chir Orthop Traumatol Cech , 2002, 69: 16-21.
  • 8John A, Hong L, Ikada Y, Tabata Y. A trial to prepare biodegradable collagen-hydroxyapatite composites for bone repair. J Biomater Sci Polym Ed, 2001, 12: 689-705.
  • 9Agrillo U, Mastronardi L, Puzzilli F. Anterior cervical fusion with carbon fiber cage containing coralline hydroxyapatite:preliminary observations in 45 consecutive cases of soft-disc herniation. J Neurosurg, 2002, 96: 273-276.
  • 10Lind M. Growth factor stimulation of bone healing. Effects on osteoblasts, osteomies, and implants fixation. Acta Orthop Scand, 1998,283 (Suppl): 32-37.

共引文献20

同被引文献63

  • 1谢剑宏,惠忠英,吴渺.羟基化碳纳米管/聚己内酯复合材料的结构和力学性能![J].湖北大学学报（自然科学版）,2012,34(4):491-495. 被引量：4
  • 2叶玲,苏勤,周学东.纳米羟磷灰石/聚酰胺66对成骨细胞生物学作用的实验研究[J].华西口腔医学杂志,2005,23(2):142-144. 被引量：6
  • 3Causa F, Netti P A, Ambrosio I., et al. Poly epsiloncaprolactone/ hydroxyapatite composites for bone regeneration in vitro eharac terization and human osteoblast response[J]. J Biomed Mater Res A,2006(76): 5162.
  • 4Owen T A, Aronow M, Shalhoub V, et al Progressive develop- ment of the rat oeob]ast phenotype in vitro.- reciprocal relaion- ships in expression of genes associated with osteoblast prolifera- tion and differentiation during formation of the bone extra cellular matrix[J]. J Cell Physio,ll 990,143 (3) =420.
  • 5Hsw F Y,Chueh S C,Wang Y J. Microshperes of hydroxyapatite/ reconstitutend eollagc as supports for osteoblast cell growth[J]. Biomaterials,1999(20) :1 931--1 936.
  • 6Wang X J, Li Y B. Development of biomineticnano--hydroxyapa- tite/poly(hexamethylethyleneadipamide) composites[J]. Bioma- terials,2002(23) :4 7874 791.
  • 7Rohner D, Hutmaeher DW, See P, et al. Individually CAD -- CAM technique designedbioresorbable 3--dimensional Polycap- rolactone frame work for experimental reconstruction of cranio- facial defeets in the pig[J]. M KG,2002,6(3) : 162167.
  • 8Jayarama Reddy Venugopal,Sharon I.ow, Aw Tar Choon. Nano- bioengineered Electrospun Composite Nanofibers and ()steo- blasts for Bone Rcgenera[J]. Artif Organs, 2008,32 (5) : 388 397.
  • 9Gisha E, et al. Journal of Polymers and The Environment, 2011, 19:637-676.
  • 10Gou Pengfei, et al. Biomacromolecules, 2010,11 (4):934-943.

引证文献2

二级引证文献12

中南民族大学学报（自然科学版）
2011年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部