痛泻要方对内脏高敏感大鼠脑干孤束核CGRP表达的影响

Expression of CGRP in the Nucleus of Solitary Tract of Visceral Hypersensitivity Rat Intervened by Tongxieyaofang Decoction

目的:观察痛泻要方对内脏高敏感模型大鼠脑干孤束核降钙素基因相关肽(CGRP)表达的影响,探讨痛泻要方治疗肠易激综合征(IBS)的作用机制。方法:将56只大鼠随机分成正常组、模型组、治疗组、对照组,每组14只,以灌服番泻叶煎剂加束缚桶限制活动的方法建立内脏高敏感模型,模型组予生理盐水灌胃,治疗组予痛泻要方水溶液灌胃,对照组予得舒特水溶液灌胃。试验结束后做内固定并取脑干,采用冰冻切片免疫组化染色,观察各组大鼠脑干孤束核CGRP的表达情况。结果:各组大鼠脑干孤束核均有CGRP表达,治疗组表达明显低于模型组(P<0.05),治疗组、对照组、正常组间差异无统计学意义(P>0.05)。结论:内脏高敏感状态下,大鼠脑干孤束核CGRP表达增加,而痛泻要方干预后其表达明显下降,提示降低CGRP在高级神经中枢孤束核的表达可能是痛泻要方治疗IBS的作用机制之一。

To observe the effect of Tongxieyaofang decoction on the expression of ealcitonin gene-related peptide （CGRP） in the nucleus of solitary tract of rats with visceral hypersensitivity. Methods： A total of 56 rats were randomized into group A （normal control）, group B, group C, and group D, 14 rats in each. Except group A, others were intragastric administered with senna and were constrained limb exercise. After visceral hypersensitivity model was established, group B was administered with saline, group C with Tongxieyaofang decoction, and group D with dicetel for 2 weeks. Then the brainstem of rats was removed and processed for detection of the expression of CGRP in the nucleus of solitary tract by using immunohistochemical assay. Results： The expression of CGRP was detected in all groups. A significant difference in the expression of CGRP was noted between group B and C（P〈0.05）, while no difference was found between group A, C, and D （P〉0.05）. Conclusion： In the condition of visceral hypersensitivity, the expression of CGRP in the nucleus of solitary tract of rats increased, and the increase can be reversed after intervention with Tongxieyaofang decoction. This indicates that one of the mechanisms involved in Tongxieyaofang decoction treating irr/table bowel ：syndrome may be decreasing the expression of CGRP in the nucleus of solitary tract.