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HPLC法测定鸡嗉子叶中3种黄酮苷的含量 被引量:3

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摘要 目的:建立鸡嗉子叶中金丝桃苷、异槲皮苷、槲皮苷的含量测定方法。方法:采用高效液相色谱法,以C18柱,甲醇-0.2%磷酸溶液梯度洗脱,检测波长352 nm,进行定量测定。结果:金丝桃苷、异槲皮苷、槲皮苷在100倍范围内线性关系良好,相关系数均大于0.9990,加样回收率分别为100.33%、97.78%、99.83%。结论:该方法能准确可靠地测定鸡嗉子叶中金丝桃苷,异槲皮苷,槲皮苷的含量。
作者 字刚 丁中涛
出处 《云南中医中药杂志》 2011年第7期72-73,共2页 Yunnan Journal of Traditional Chinese Medicine and Materia Medica
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