气体信号分子硫化氢对高脂饲养大鼠肝脏脂代谢的调节作用

Effects of hydrogen sulfide on lipid metabolism in liver of high-fat-fed rats

目的探讨气体信号分子硫化氢(H2S)对高脂饲养大鼠肝脏脂代谢的调节作用。方法雄性SD大鼠24只,随机分为对照组、高脂组和高脂+NaHS组,每组8只。喂养8周后处死大鼠,留取肝组织冰冻切片,分别进行油红O染色、总胆固醇PHILIPIN染色及HE染色,对油红O染色和总胆固醇染色结果采用积分光密度计数进行图像分析。结果经油红O染色后在光镜下观察,对照组肝组织的细胞形态和结构正常;高脂组肝细胞和细胞间隙有脂质大量弥漫沉积,肝小叶结构破坏;高脂+NaHS组脂质沉积(5818.79±297.45)较高脂组(62 612.70±756.46)明显减少(P<0.01),肝小叶结构破坏程度减轻。对照组总胆固醇PHILIPIN荧光染色无阳性信号显示;高脂组总胆固醇PHILIPIN荧光染色(201.21±3.18)呈明蓝色荧光阳性信号,信号强烈且分布较均匀;高脂+NaHS组明蓝色荧光阳性信号强度(52.13±3.70)明显减弱,与高脂组比较差异有统计学意义(P<0.01)。HE染色光镜下观察见对照组肝细胞形态和结构正常;高脂组大量肝细胞含有脂肪滴,将胞核推挤到细胞一侧,肝细胞肿大变圆,小叶中央区受累明显;高脂+NaHS组肝细胞内脂肪滴明显减少,肝细胞形态和结构有所恢复。结论外源性H2S对大鼠高脂饮食所致肝脏脂质代谢异常有明显改善作用。

Objective To investigate the effects of hydrogen sulfide on lipid metabolism in liver of high-fat-fed（H-FD） rats.Methods Twenty-four male SD rats were randomly divided into control group,H-FD group and H-FD＋NaHS group（8 each）.Rats were sacrificed after feeding for eight weeks,and hepatic tissue was obtained and frozen sectioned.They were stained with oil red O,Philipin and HE.The oil red O and Philipin-dyed pictures were measured by image analysis system.Results Oil red O staining showed that the cell shape and structure of hepatic tissue were normal in control group,and massive diffuse lipid deposition and damaged structure in hepatic lobule were found in H-FD group.The lipid deposition decreased significantly,and the damage of hepatic lobule also ameliorated,in H-FD＋NaHS group（5818.79±297.45） compared with H-FD group（62612.70±756.46,P〈0.01）.Philipin staining showed that the fluorescence signal of total cholesterol was negative in control group,and it was positive in H-FD group where the signal was strong and well distributed.The fluorescence intensity of total cholesterol was significantly weakened in H-FD＋NaHS group（52.13±3.70） compared with H-FD group（201.21±3.18,P〈0.01）.Moicroscopic examination with HE staining showed that the cell shape and structure of hepatic tissue were normal in control group;the hepatocytes contained big lipid droplets,becoming swollen and rounded with obviously injured central area of hepatic lobule in H-FD group;while the lipid droplets in hepatocytes was distinctly decreased in H-FD＋NaHS group,and the shape and structure of hepatocytes recovered to certain extent.Conclusion Exogenous hydrogen sulfide might show a novel regulatory effect on abnormality of lipid metabolism in liver of high-fat-fed rats.