PTTG、bFGF、VEGF-C在大肠正常黏膜及大肠癌中的表达及意义

Expression and Clinical Significance of PTTG, bFGF, and VEGF-C in Normal Mucosa and Cancer of the Large Intestine

目的:探讨垂体肿瘤转化基因(PTTC)、碱性成纤维细胞生长因子(bFCF)、血管内皮生长因子-C(VECF-C)在大肠癌中的表达及以上指标与大肠癌临床病理因素之间的关系。方法:采用RT-PCR技术检测20例大肠正常黏膜、80例大肠癌组织中PTTC mRNA,应用免疫组织化学法检测20例大肠正常黏膜、80例大肠癌组织中PTTC、bFCF、VECF-C蛋白的表达。结果:大肠癌组织中PTTC、bFCF、VECF-C的表达明显高于大肠正常黏膜组织,且与大肠癌浸润深度、淋巴结转移、Duke’s分期呈正相关,PTTC、bFCF、VECF-C在大肠癌中的表达有显著正相关性。结论:PTTC、bFCF、VECF-C在大肠癌发生、发展、侵袭及转移过程中扮演着重要的角色,可作为判断大肠癌转移及预后的预测因素,且三者可能通过相互作用共同促进大肠癌的恶性进展。

Objective： investigate the expression and clinical significance of the pituitary tumor transformed gene （ PTTG ）, the basic fibroblast growth factor （ bFGF ）, and the vascular endothelial growth factor-C （ VEGF-C ） in the occurrence and progression of human colorectal carcinoma （ CC ）. The relationship between these indices and clinicopathologic factors was also examined. Methods： Reverse transcriptase-polymerase chain reaction （ RT-PCR ） was used to determine the mRNA expression of PTTG. Streptavidin peroxidase （ SP ） immunohistochemical staining was performed to assay the expression of the three proteins in 20 normal mucosa （ NM ） and 80 CC tissues. Results： The PTTG, bFGF, and VEGF-C expression levels in CC were significantly higher than those in NM （ P 〈 0.01, P 〈 0.01, and P 〈 0.01, respectively ）, and were positively correlated with the depth of tumor invasion, lymph node metastasis, and Duke＇s staging. Significantly positive correlations were observed among PTTG, b-FGF, and VEGF-C in CC tissues. Conelusion： The high PTTG, bFGF, and VEGF-C expression may be significantly involved in the onset and progression of CC, and can be used as a predictive and prognostic factor. Interactions between PTTG, bFGF, and VEGF-C may promote CC progression.