间质性膀胱炎肥大细胞雌激素受体的表达及意义

Expression and Significance of Estrogen Receptor in Bladder Mast Cells in Patients with Interstitial Cystitis

目的:检测雌激素受体在间质性膀胱炎(IC)患者膀胱黏膜肥大细胞上的表达情况,探讨雌激素通过调控肥大细胞的激活在IC发病机制中的作用及其临床意义。方法:IC患者膀胱黏膜活检组织标本18例,12例正常膀胱黏膜活检组织标本作为对照组。分别采用0.5%甲苯胺蓝及抗人肥大细胞类胰酶抗体标识组织标本肥大细胞;多克隆抗人雌激素受体抗体标识标本膀胱黏膜上雌激素受体;分析2组标本在肥大细胞数目、脱颗粒肥大细胞比例及雌激素受体表达方面的差异。结果:IC组织标本中肥大细胞密度4.38(3.96,5.29)个/HP,明显高于对照组的2.30(2.26,2.40)个/HP,差异有统计学意义(Z=2.561,P<0.05);IC组织中75.3%的肥大细胞呈不同程度脱颗粒状态,对照组未见明显脱颗粒肥大细胞存在;IC组织标本中雌激素受体阳性肥大细胞(ER+)3.48(3.16,3.69)个/HP,对照组ER+0.82(0.79,0.86)个/HP,2组比较差异有统计学意义(Z=2.988,P<0.05);IC组织中肥大细胞数与ER+数呈正相关(r=0.884,t=3.870,P<0.01)。结论:雌激素可能通过其在肥大细胞上的受体表达来调控肥大细胞脱颗粒,产生IC特有的临床症状。

Objective：To investigate the role of estrogen and the mast cells in the pathogenesis of the interstitial cystitis（IC）,specifically with respect to the activated bladder mast cells.Methods：Eighteen cystoscopy biopsy specimens from patients with interstitial cystitis and 12 control subjects were studied in this study.Mast cells were stained by 0.5% toluidine blue and immunohistochemical methods.And double immunohistochemical staining was used to manifest the expression of estrogen receptor（ER） in mast cells of interstitial cystitis.Results：The mast cell density of interstitial cystitis was 4.38（3.96,5.29）/HP,which was significantly higher than that of control group [2.30（2.26,2.40）,P 〈 0.05].There was 75.3% mast cell degranulation in interstitial cystitis compared with non in control group.There were significant differences in ER＋cells [3.48（3.16,3.69）/HP] in interstitial cystitis group and control group [0.82（0.79,0.86）/HP,P 〈 0.05].The number of mast cells was positively correlated with the ER＋cells in IC（P 〈 0.05）.Conclusion：Estrogen may regulate the mast cell degranulation through the receptor expression in mast cells,which showing the clinical symptoms of interstitial cystitis.