基于荧光图像的抗肿瘤细胞迁移药物筛选方法

Development of fluorescence imaging based assay for screening compounds with anti-migration activity

本文建立了一种基于荧光图像的抗肿瘤细胞迁移药物筛选方法。应用Hoechst 33342荧光染料染色和96孔Transwell板,通过细胞荧光图像采集与分析平台对正常组和加药组细胞荧光图像进行定量分析,建立了一种快速筛选抗肿瘤细胞迁移药物的新方法。对本方法的稳定性、精密度、荧光染色时间和迁移时间进行了考察和优化,并对抗肿瘤药紫杉醇的肿瘤细胞迁移抑制作用进行了定量分析,计算得到紫杉醇抗肿瘤细胞迁移的IC50为0.717μmol.L-1。采用该方法对中药泽泻24个组分进行筛选,发现1个活性组分。结果表明,该方法快速、准确、重现性好,能够用于抗肿瘤细胞迁移药物的快速筛选。

In the present study,A fluorescent imaging-based high-throughput screening method was developed for identifying anti-migratory compounds with 96-well Transwell plates.The correlation,precision and stability of this method were examined and the incubation time of dye Hoechst 33342 in addition to migration time was optimized.In addition,The inhibitory activity of anti-cancer drug paclitaxel on tumor cell migration was assayed and an IC50 value of 0.717 μmol·L-1 was obtained.Using this method,24 components from Rhizoma Alismatis were screened and one component with anti-migration activity was found.These results show that the new proposed method with good precision,stability and linear range has the potential to assay the inhibitory activity of anticancer compounds.