一株淮山炭疽病拮抗放线菌的分离及初步鉴定

Isolation of an Antagonistic Actinomycete Strain against Dioscorea opposita Thunb Anthracnose and Its Primary Identification

[目的]从海南药用植物根际分离得到对淮山炭疽病具有拮抗活性的放线菌菌株,并对其分类地位进行鉴定。[方法]采用HV培养基从盾叶鸡蛋花根际土壤分离放线菌菌株;通过平板对峙培养和发酵代谢产物抑菌试验筛选活性放线菌;根据形态特征,结合16SrRNA基因序列分析鉴定活性放线菌的分类地位。[结果]从盾叶鸡蛋花的根际土壤分离到12株放线菌,筛选到1株对淮山炭疽病菌具有良好拮抗活性的菌株51173,菌株51173鉴定为链霉菌属Streptomyces violaceusniger16S rRNA gene clade中的成员。[结论]放线菌菌株51173在淮山炭疽病菌的生物防治中将具有很好的开发应用前景。

[Objective]The paper was to isolate actinomycete strains with antagonistic activity against Dioscorea opposita Thunb anthracnose from the rhizosphere soil of medicinal plants in Hainan Province,and identify its taxonomic status.[Method]Using HV medium,actinomycete strains were isolated from the rhizosphere soil of frangipani（Plumeria rubra L.）.The active antagonistic actinomycetes were selected by using confrontation culture method and inhibition test of fermentation products.According to morphology,combined with 16S rRNA gene analysis,the taxonomic status of active actinomycetes was identified.[Result]Twelve strains of actinomycetes were isolated from the rhizosphere soil of medicinal plant frangipani（P.rubra L.）.Strain 51173 showed significant antifungal activity against Colletotrichum gloeosporioides,which was identified to be a member of Streptomyces violaceusniger 16S rRNA gene clade.[Conclusion]Strain 51173 showed a good application potential for biological control against D.opposita Thunb anthracnose.