摘要
目的:探讨抗-HCV与HCV-RNA的相关性及临床应用价值。方法:ELISA方法检测抗-HCV,实时荧光定量PCR检测HCV-RNA。结果:检测可疑HCV感染者156例,其中119例抗-HCV阳性,同时HCV-RNA阳性为73例。HCV-RNA阳性患者中抗-HCV的阳性率显著高于抗-HCV阳性患者中HCV-RNA的阳性率(P<0.05)。结论:同时检测抗-HCV和HCV-RNA可提高HCV感染诊断的阳性率,检测HCV-RNA对抗病毒治疗的疗效评价及治疗时间有重要意义。
Objective：To elevate the sensitivity of hepatitis C virus（HCV）and anti-HCV detection for diagnosis of HCV infection.Method：Real-time fluoresce quantitative reverse transcription polymerase chain reaction（FQ-RT-PCR）was used to detect HCV-RNA in serum,and ELISA was used to detect anti-HCV.Results：The anti-HCV of 119samples showed positive test and the HCV-RNA of 73showed positive test in 156patients possibly infected with HCV.The anti-HCV positive frequency（98.6%）in HCV-RNA positive patients was significant higher than the HCV-RNA positive frequency（60.8%）in anti-HCV positive patients（P0.05）.Conclusion：Simultaneous detection of both anti-HCV and HCV-RNA can elevate the positive frequency of HCV infection,and the detection of HCV-RNA is helpful in the diagnosis and treatment HCV infection.
