二甲亚砜抑制肿瘤坏死因子刺激内皮细胞粘附分子的表达

Dimethyl Sulfoxide Inhibits the Expression of Adhesive Molecules on Endothelial Cells Stimulated by Tumor Necrosis Factor-α

为了探讨抗氧化剂二甲亚砜对白细胞粘附肿瘤坏死因子－α活化的人脐静脉内皮细胞，及其对肿瘤坏死因子－ α刺激的内皮细胞表达粘附分子、Ｅ－ 选择素、细胞间粘附分子－１ 的影响，用酶联免疫吸附分析方法检测内皮细胞的粘附分子表达，应用孟加拉玫红活细胞染色测定白细胞与内皮细胞的粘附。结果发现， 用肿瘤坏死因子－ α刺激人脐静脉内皮细胞６ ｈ显著增加白细胞与内皮细胞的粘附（ Ｐ＜０．０１）；用肿瘤坏死因子－ α孵育人脐静脉内皮细胞４ ｈ 或１８ ｈ 可明显诱导Ｅ－ 选择素的表达和增加细胞间粘附分子的构成表达（Ｐ＜ ０ ．０１） ；用２ ．５ ％ 二甲亚砜预孵育内皮细胞１ ｈ 再与肿瘤坏死因子－ α共同孵育细胞明显减少白细胞与内皮细胞的粘附， 并抑制内皮细胞表达Ｅ－ 选择素和细胞间粘附分子－ １（ Ｐ＜ ０．０１）。以上提示抗氧化剂二甲亚砜可抑制肿瘤坏死因子－α活化的人脐静脉内皮细胞表达Ｅ－ 选择素和细胞间粘附分子－ １，减少白细胞与活化内皮细胞的粘附，

Aim To investigate the effects of dimethyl sulfoxide (DMSO), an antioxidant, on neutrophils adhesion to tumor necrosis factor-α (TNF-α)-activated human umbilical vein endothelial cells (HUVEC), and the expression of adhesion molecules E-selectin and intercellular adhesion molecule-1 (ICAM-1) on TNF-α-stimulated endothelial cells. Methods The expression of adhesion molecules on HUVEC was measured by a cellular enzyme-linked immunoabsorbent assay(ELISA)method,and the adherence of neutrophils to activated HUVEC was detected by Rose Bengal assay. Results After stimulation of HUVEC with TNF-α for 6 h, the adherence of neutrophils to endothelial cells was increased compared with control group (P<0.01). Exposure HUVEC to TNF-α for 4 h or 18 h induced E-selectin expression and increased constitutive expression of ICAM-1 (P<0.01). Pretreatment of HUVEC with 2.5% DMSO for 1 h before stimulation with TNF-α produced a decrease in the neutrophil adhesion and inhibition in the expression of E-selectin and ICAM-1 (P<0.01). Conclusion DMSO decrease neutrophil adhesion to TNF-α-activated endothelial cell by inhibiting the expression of E-selectin and ICAM-1. This result may contribute to the anti-inflammatory and antiatherosclerotic potential of DMSO.