抗氧化剂对脂质过氧化内皮细胞表达细胞粘附分子的影响

Effect of Antioxidant on the Expression of Cellular Adhesion Molecules in Cultured Endothelial Cells Induced by Peroxidation

为探讨抗氧化剂维生素Ｅ和元素硒对脂质过氧化诱导内皮细胞表达细胞粘附分子及单核细胞粘附的影响，用联胺诱发培养内皮细胞脂质过氧化，检测维生素Ｅ和元素硒对单核细胞粘附的影响，用免疫组化及共焦显微镜观察维生素Ｅ和元素硒对内皮细胞表达血管细胞粘附分子１ 及内皮细胞白细胞粘附分子１ 的影响。结果显示抗氧化剂维生素Ｅ和元素硒可抑制内皮细胞脂质过氧化，抑制血管细胞粘附分子１ 及内皮细胞白细胞粘附分子１ 表达，减少单核细胞粘附。提示抗氧化剂有可能通过抑制内皮细胞表达细胞粘附分子。

Aim To investigate the effect of antioxidant Vitamine E and Selenium on the expression of cellular adhesion molecules(CAMs) induced by lipid peroxidation(LPx) in cultured endothelial cells and monocytes(MC) adherence to EC. Methods LPx was initiated withdiamide(DM).Effect of Vitamine E and Selenium on the expression of vascular adhesion molecule-1(VCAM-1) and endothelial leucocyte adhesion molecule-1(ELAM-1) was studied under laser confocal microscope,MC adherence to EC was measured. Results Vitamine E and Selenium could decrese the content of lipid peroxid,reduce VCAM-1 and ELAM-1 expression andMC adherence to EC incubated with DM. Conclusion Antioxidant could inhibite VCAM-1 and ELAM-1expression induced by LPx to reduce Mc adherence to EC and may possiboly prevent the development of athrosclerosis (As) lesion.