骶骨脊索瘤CD40、PCNA、CD31表达及其与肿瘤复发的相关性研究

Expressions of CD40,PCNA,CD31 in human chordoma and their relationship with recurrence of chordoma

[目的]研究骶骨脊索瘤中CD40、PCNA、CD31的表达水平及其与脊索瘤复发的相关性。[方法]采用EnVi-sionTM免疫组化技术检测28例骶骨脊索瘤组织、9例正常胚胎脊索组织中的CD40和PCNA的表达,并计数CD31标记的肿瘤组织微血管密度(MVD)。[结果]①CD40、PCNA在骶骨脊索瘤中的强阳性表达率分别为66.1%、64.3%,明显高于正常胚胎脊索组织。两者之间的差异有统计学意义(P<0.05);骶骨脊索瘤中CD31标记的微血管密度(24.26±18.68)/mm2,正常胚胎脊索组织微血管密度值为(4.63±2.19)/mm2;差异有统计学意义(P<0.05);②28例骶骨脊索瘤,其中复发组14例,非复发组14例,两组间CD40阳性表达率分别为14.29%、85.71%;PCNA阳性表达率分别为92.86%、50.00%;CD31标记的MVD值分别为(30.66±23.75)/mm2、(17.88±8.52)/mm2;均有统计学差异(P<0.05);③CD40、PCNA表达及CD31标记的MVD值与患者的年龄、性别、肿瘤大小无统计学相关性;④PCNA阳性表达的骶骨脊索瘤组织中MVD值为(28.92±20.27)/mm2,明显高于PCNA阴性组(12.64±3.70)/mm2,差异有统计学意义(P<0.05)。CD40阴性表达的骶骨脊索瘤组织中MVD值为(31.68±22.81)/mm2,明显高于CD40阳性表达组(16.86±9.28)/mm2,差异有统计学意义(P<0.05)。[结论]骶骨脊索瘤中CD40、PCNA、CD31表达水平可作为判断病情进展和肿瘤复发的指标。

[Objective]To study the expressions of CD40,PCNA and CD31 in human chordoma tissues and the relationship with the recurrence of chordoma. [Methods]CD40,PCNA and CD31 were detected by EnVisionTM immunohistochemistry method in 28 human chordoma tissues and 9 normal notochord tissues.The tumor microvascular density（MVD） marked by CD31 were counted.[Results]①The positive expression rates of CD40 and PCNA in chordoma were 66.1% and 64.3%.They were significantly higher than those in the normal notochord tissues（P0.05）.The MVD marked by CD31 was 24.26±18.68/mm2 in the chordoma tissues and was 4.63±2.19/mm2 in the normal notochord tissue.There was significant difference（P0.05）.②Of the 28 chordoma patients,14 were diagnosed with recurrence.The positive expressions of CD40 in the recurrence group and non-recurrence group were 14.29% and 85.71% and 92.86% and 50.00% for the PCNA.The CD31 marked MVD was 30.66±23.75/mm2 in the recurrence group and 17.88±8.52/mm2 in the non-recurrence group.There was significantly difference between the two groups（P0.05）.③There was no statistical relationship between the expression of CD40,PCNA,MVD versus age,gender,and tumor size.④ The MVD in the positive expression group of PCNA was 28.92±20.27/mm2.It was significantly higher than that in the negative expression group（12.64±3.70 /mm2）（P0.05）.The MVD in the negative expression group of CD40 was 31.68±22.81/mm2.It was significantly higher than that in the positive expression group（16.86±9.28/mm2）（P0.05）.[Conclusion]The expression of CD40,PCNA and CD31 in chordoma could be used to estimate the progression and recurrence.