摘要
通过观察TLR2蛋白阻断前后HCV阳性血清处理BV-2细胞培养上清液中MCP-1、IL-6及IL-12的表达,为进一步探讨HCV引起中枢神经系统免疫反应特点奠定基础。细胞分为空白对照组(常规培养用DMEM高糖培养液)、正常对照组(含有20%正常血清的DMEM高糖培养液)、实验组(含有20%HCV阳性血清的DMEM高糖培养夜),每组分为4 h、8h、16 h及24 h,同时设24 h阻断组(加TLR2单克隆抗体孵育30 min后给予含有20%HCV阳性血清的DMEM高糖培养液培养24 h),采用ELISA方法检测各组各时间点培养上清液中MCP-1、IL-6及IL-12的表达水平,并使用SPSS13.0统计软件对结果进行统计分析,结果发现HCV阳性血清处理后MCP-1、IL-6及IL-12的表达显著增加,与空白对照及正常血清对照组比较具有统计学意义(P<0.05);TLR2蛋白阻断后MCP-1、IL-6及IL-12的表达明显下降,与实验组24 h比较具有统计学意义(P<0.05)。本研究发现,HCV阳性血清处理后TLR2介导的MCP-1、IL-6及IL-12的表达明显增加,TLR2可能通过激活下游大量炎性因子参与BV-2抗HCV免疫。
To observe the expression of MCP-1,IL-6 and IL-12 on the cultural supernatants of the mouse BV-2 cells treated with HCV-positive serum before and after blockage with TLR2 protein for the further investigation on the immune response characteristics in central nervous system,cells were divided into three groups.i.e.control group（regular DMEM high glucose medium）,normal group（20% normal serum containing DMEM high glucose medium） and the experimental group（with 20% HCV-positive serum DMEM high glucose medium）.The TLR2 blocked group was incubated with TLR2-monoclonal antibody for 30 min and then cultured with 20% HCV positive serum in DMEM high glucose medium for 24 hrs.ELISA was used to detect the expression of MCP-1,IL-6 and IL-12 in the infected BV-2 cells 4,8,16 and 24 hrs after HCV infection.It was demonstrated that the expression of MCP-1,IL-6 and IL-12 was significantly increased after stimulation with HCV-positive serum,and significantly decreased after TLR2 blockage.There were significant difference between control group,normal group and experiment group（P0.05）.It is apparent that TLR2-mediated MCP-1,IL-6 and IL-12 expression in BV-2 cells after HCV-positive serum stimulation might be involved in the anti-HCV immunity.
出处
《现代免疫学》
CAS
CSCD
北大核心
2011年第4期321-324,共4页
Current Immunology
基金
宁夏医科大学博士点建设开放课题(KF2010-34)
