一色齿毛菌漆酶—介体系统在染料脱色中的应用

Application of Cerrena unicolor laccase-mediator system in dye decolorization

本文通过一色齿毛菌LS0547漆酶粗酶液与紫脲酸(VA)或乙酰丁香酮(Ace)组成的漆酶--介体系统对结晶紫、靛红、活性黑KN--B染料进行脱色试验,探索pH、温度、染料浓度和漆酶浓度等条件对其脱色效果的影响。结果表明:在介体乙酰丁香酮(Ace)作用下,漆酶脱色靛红和活性黑KN--B效率高;在介体紫脲酸(VA)作用下结晶紫脱色效果好。pH4.0、50℃结晶紫的脱色率为95.42%,40℃靛红脱色率为99.86%,且基本不受pH限制;pH6.0、40℃活性黑KN--B脱色率达92.38%,表明一色齿毛菌漆酶在介体参与下可有效用于工业染料废水的处理。

In this study, we investigated the decolorization of the crystal violet, indigo carmine and reactive black KN-B by a laccase-mediator system. The mediator system was composed of laccase from Cerrena unicolor LS0547 and the mediator of violuric acid （VA） or acetosyringone （Ace）. The effects of initial pH, temperature, dye concentration and the dosage of laccase on the decolorization of dyes were tested. The results showed that the decolorization of indigo carmine and reactive black KN-B was remarkably improved in the presence of Ace, while crystal violet was more efficiently decolorized with the addition of VA. The decolorization rate of crystal violet reached 95.42% at pH 4.0 and 50℃. Indigo carmine could be decolorized with a rate of 99.86% at 40 ℃ , which was not affected by pH. And 92.38% of reactive black KN-B was decolorized at pH 6.0 and 40℃. Therefore, the C. unicolor laccase-mediator system has a potential application in the treatment of industrial dye wastewater.