酶法制备谷胱甘肽工艺的研究

Study on Enzymatic Synthesis of Glutathione

以含有ｒ － 谷氨酰半胱氨酸合成酶（ ＧＳＨＩ） 和谷胱甘肽合成酶（ ＧＳＨⅡ） 的Ｓ－ｃｅｒｅｖｉｓｉａｅｅｍｕｌｓｅｆｉｅｒ－ Ｅ４７２ｃ 酵母菌株为酶源， 以谷氨酸、半胱氨酸、甘氨酸为底物， 酶法制备谷胱甘肽（ＧＳＨ） 工艺的研究。采用了直接高效液相色谱法检测酶促反应中ＧＳＨ 的含量， 建立了酶促反应的最佳反应条件。在优化反应条件下， 对底物半胱氨酸的转化率为８０ ％ ，经过分离纯化回收率为８３ ％ ，总产率为６６－４ ％ 。结果表明， 该法制备ＧＳＨ 成本低， 工艺过程简单， 能够适用于ＧＳＨ 的工业化生产。

A simple and economical method of enzymatic synthesis of Glutathione was developed Glutathione was produced by means of the biotransformation of L Glutamic acid L Cysteine and Glycine in the presence of yeast cell of S cerevisiae emulsefier E 472c The analytical method of determine GSH and GSSG with HPLC was founded and a series reaction conditions were optimized Under this condition, the transformation rate can approach 80% with a mole yield of 82%.