卡介苗素抗哮喘有效成分的分离及其药理作用研究

Isolation and Purification of Antiasthma Composition from BCG PSN and Study on Its Pharmacological Properties

卡介苗素具有平喘作用，主要含多糖和核酸两种组分，为明确其有效成分，将卡介苗素经核酸酶酶解，去除其中的核酸组成后，经Ｓｅｐｈａｄｅｘ Ｇ１００ 柱层析分离纯化，得到一种白色粉末状多糖———卡介菌多糖（ＢＣＧＰＳＡ） 。该多糖经聚丙烯酰胺凝胶电泳，Ｓｅｐｈａｄｅｘ Ｇ２００ 柱层析证实为均一成分。对ＢＣＧＰＳＡ的化学组成分析表明，该多糖的分子量为１－７０５ ×１０５ ，不含硫酸基和糖醛酸。经纸层析、薄层层析及气相层析确证，其单糖组成仅为Ｄ葡萄糖，属均一多糖类。对ＢＣＧＰＳＡ 的药理研究表明，该多糖具有明显的免疫调节功能和平喘作用，且毒性很小，是卡介苗素抗哮喘的有效成分。

The purification of BCG polysaccharide nucleic acid (BCG PSN) was described in this paper Nucleic acid and protein composition was removed with nuclease and trichloroacetic acid butanol respectively A white powder was got by Sephadex G 100 column chromatography This white powder was BCG polysaccharide (BCG PSA) Examined with polyacrylamide gel electrophoresis and Sephadex G 200 column chromatography, the BCG PSA was homogeneous The analysis in BCG PSA's chemical composition showed that its molecular weight was 1 705×10 5 Its monosaccharide composition was D glucose only confirmed by paper chromatography, TLC and gas chromatography