乙型肝炎病毒DNA在肝癌患者基因组中的检测

Detection of HBV DNA integrated in genome of liver neoplasm with PCR technique.

目的：为了研究乙型肝炎病毒ＤＮＡ在人类基因组中的整合作用与肝癌发生之间的关系。方法：采用聚合酶链反应技术，对肝癌细胞基因组中的乙肝病毒ＤＮＡ第Ｖ 区段进行了测定。结果：１２ 例肝癌细胞基因组８ 例为阳性，１０ 例肝癌患者外周血细胞基因组均为阴性。结论：乙型肝炎病毒ＤＮＡ 的整合与肝癌发生有密切关系。

Objective:In order to investigate the relationship between the occurrence of liver neoplasm and integration of HBV DNA in human genome. Methods:PCR Technique was used to detect the sequence V of HBV DNA in the liver neoplasm genome and human genome of peripheral blood cells. Resnlts :8 of 12 liver neoplasm were positive, the all genomes of 10 peripheral blood with liver neoplasm were negative. Conclusion: There is a close relation between the integration of HBV DNA and occurrence of liver neoplasm.