应用PCR技术对孕妇HCMV感染的前瞻性研究

Prospective study on the HCMV infection in pregnant women by PCR.

应用聚合酶链反应（ＰＣＲ）技术对１５６ 例孕妇尿中ＨＣＭＶ 进行检测，并追踪观察１１４ 例新生儿脐血中ＨＣＭＶ 垂直传播情况。结果表明：１１ 例孕妇尿ＨＣＭＶＤＮＡ 检测阳性，孕期感染率为７－０５％ ，有异常妊娠史孕妇感染率（１４－７１ ％ ）较正常孕妇（４－９２ ％） 明显升高。９ 例ＨＣＭＶ 阳性孕妇有４ 例观察新生儿脐血ＨＣＭＶＤＮＡ 阳性，占４４％ ，而ＨＣＭＶＤＮＡ阴性孕妇无１ 例新生儿脐血出现阳性结果。我们认为ＰＣＲ方法是检测ＨＣＭＶ宫内感染的可靠而灵敏的指标，对ＨＣＭＶＤＮＡ阳性的孕妇应进一步检查羊水，如羊水ＨＣＭＶＤＮＡ也阳性，应终止妊娠。

HCMV in the urine from 156 pregnant women was determined by PCR and a tracing study on the vertical transmission of HCMV in cord bood was made on 114 new-borns. Results were as following. HCMV-DNA in the urine from 11 pregnant women was pastive and the infection rate was 7.05%; the infection rate of pregnant women with a history of abnormal pregnancy was 14,71%, which was much highter than that of normal pregrant women; among 9 pregnant women with positive HCMV, 4 had positive HCMV-DNA in cord blood of new-borns(44%)while there was no positive HCMV-DNA in cord blood of new-borns in pregnant women with negative HCMV.We think that PCR is a quick and reliable index in detecting intranterine HCMV infection. The pregnant women with positive HCMV-DNA should also have amniotie fluid examined. If HCMV-DNA in the amniotic fluid is also positive, the pregnancy should be terminated.