白藜芦醇对大鼠缺血再灌注损伤后脑组织的保护作用

Neuroprotective effect of resveratrol on cerebral tissues after ischemia-reperfusion injury and its mechanisms in rats

目的探讨白藜芦醇对大鼠缺血再灌注损伤后脑组织的保护作用及其机制。方法利用线栓法制作大鼠脑缺血再灌注损伤模型。72只SD大鼠按随机数字表法随机平均分为假手术组、对照组、白藜芦醇高剂量组和白藜芦醇低剂量组。缺血2h再灌注24h后,分别测定动物的神经损伤功能评分、脑组织梗死体积,缺血再灌注损伤的脑组织中髓过氧化物酶(MPO)的活性、伊文思兰的含量、肿瘤坏死因子-α(TNF-α)的含量及基质金属蛋白酶-9(MMP-9)的表达水平。结果白藜芦醇治疗组神经功能损伤评分均较对照组明显降低(P<0.05),脑梗死体积明显缩小(P<0.05),MPO的活性、伊文思兰的含量、TNF-α的含量及MMP-9表达水平均也明显低于对照组(P<0.05)。结论白藜芦醇可能通过降低炎症反应和血脑屏障通透性对大鼠脑缺血再灌注损伤的脑组织起神经保护作用;其抗炎作用可能与其降低TNF-α的含量有关,而降低血脑屏障通透性则可能与MMP-9的表达下调有关。

Objective To study the neuroprotective effect of resveratrol on the cerebral tissues after ischemia-reperfusion injurya nd its mechanisms in rats.Methods Seventy-two adult rats were randomly divided into sham operation,high dose（40 mg/kg·bw）r esveratrol treatment,low dose（8 mg/kg·bw）resveratrol treatment and control groups of 18 animals each.The cerebral ischemia-r eperfusion injury models were made in all the group expert the sham operation group.Neurological defect scores and cerebral infarctf oci volumes were determined 24 hours after the cerebral reperfusion.Myeloperoxidase（MPO）activities,the contents of Evans blue,w hich was injected into the vein 23 hours after the cerebral reperfusion,and tumor necrosis factor-α（TNF-α）,expression levels ofm atrix metalloproteinase-9（MMP-9）protein in the cerebral tissues,24 hours after the cerebral reperfusion injury were determined.R esults The neurological defect scores,volume of the cerebral infarct foci,and MPO activeities,Evans blue contents and expressionl evels of MMP-9 in the cerebral tissues were significantly lower in both the resveratrol treatment groups than those in the control group（ P0.05）,and they were dose-depedent.The content of TNF-αin the cerebral tissues was significantly lower in high dose resveratrolt reatment group than those in low dose treatment and control group（P0.05）.Conclusions It is suggested that resveratrol is ofn europrotective effect on the cerebral tissues after ischemia-reperfussion injury probably by the decrease in the ischemia-reperfusion-i nduced imflammatory reaction and high permeability of blood-brain barrier.The anti-imflammatory effect of resveratrol may be relatedt o the reveratrol-induced decrease in the content of TNF-αin the cerebral tissues after the cerebral ischemia-reperfusion.The decreasei n the permeability of blood-brain barrier induced by resveratrol may be related to down-regulation of the MMP-9 expression in thec erebral tissues after the cerebral ischemia-reperfusion.