NF-κB/p65和VEGF在卵巢上皮性肿瘤的表达及临床意义

Expression and clinical significance of NF-κB/p65 and VEGF in ovarian epithelial tumor.

目的:研究NF-κB/p65和VEGF在卵巢上皮性肿瘤的表达及临床意义。方法:应用免疫组化SP法,检测NF-κB/p65和VEGF在10例正常卵巢组织,20例卵巢良性上皮性肿瘤,10例卵巢交界性上皮性肿瘤,60例卵巢上皮性癌中的表达。结果:NF-κB/p65和VEGF在卵巢上皮性癌中的表达率均高于卵巢交界性上皮性肿瘤、卵巢良性上皮性肿瘤的表达率,差异有统计学意义(P<0.05)。卵巢上皮性癌中NF-κB/p65和VEGF的阳性表达率,中低分化组高于高分化组,有淋巴结转移组高于无淋巴结转移组,差异均有统计学意义(P<0.05);Ⅲ～Ⅳ期的表达率显著高于Ⅰ～Ⅱ期,差异有显著统计学意义(P<0.01)。卵巢上皮性癌患者腹水量>500m l组中VEGF表达率显著高于腹水量<500m l组(P<0.01),NF-κB/p65在腹水量>500m l组和腹水量≤500m l组中的表达率差异无统计学意义(P>0.05);VEGF与NF-κB/p65在卵巢肿瘤组织中的表达强度呈正相关(r=0.279,P<0.01)。结论:NF-κB/p65及VEGF与卵巢上皮性癌的发生发展有关,与卵巢上皮性癌病理分级、临床分期、淋巴结转移有关,VEGF与腹水形成有关;VEGF可作为判断预后的候选指标;NF-κB/p65对VEGF有调控作用。

Objective：To investigate the clinical significance and prognostic value of the expression of NF-κB/p65 and VEGF in ovarian epithelial tumors.Methods：The expression of NF-κB/p65 and VEGF in 60 cases of ovarian epithelial cancers,20 benign ovarian tumors,10 borderline epithelial tumors of the ovary and 10 normal controls were determined by using immunohistochemistry.Results：The positive expression of NF-κB/p65 and VEGF in ovarian epithelial cancers was significantly higher than that in ovarian epithelial borderline tumors and ovarian epithelial benign tumors（P0.05）;the positive rate and overexpression of NF-κB/p65 and VEGF in moderately,poorly differentiated tissues,in lymphoid metastasis and in Ⅲ～Ⅳ stages were significantly higher than that in well differentiated tissues（P0.05）,in unlymphoid metastasis ones（P0.05） and inⅠ~Ⅱ stages of ovarian epithelial cancers（P0.01）,respectively.The positive expression rate of VEGF in volume of ascites more than 500ml group was significantly higher than that in less than 500ml group of ovarian epithelial cancers（P0.01）;NF-κB/p65 expression had no significant difference between those two groups（P0.05）.There was a positive correlation between VEGF and NF-κB/p65（r=0.279,P0.01）.Conclusions：NF-κB/p65 and VEGF expression has relation with the development of ovarian epithelial cancer,pathological grades,clinical stage,and lymphoid metastasis.Under the regulation of NF-κB/p65,VEGF has also relation with the growth of ascites and maybe play an important role in prognosis indicator.