TGF-β1对糖尿病大鼠深Ⅱ度烫伤创面愈合的影响

Effects of TGF-β1 on wound healing in diabetic rats with second-degree scald

目的观察转化生长因子-β1(TGF-β1)在糖尿病大鼠深Ⅱ度烫伤创面组织中的表达,探讨外源性TGF-β1应用对创面愈合的影响。方法 18只SD大鼠随机分为单纯烫伤组(对照组)、糖尿病烫伤组(糖尿病组)和糖尿病烫伤TGF-β1干预组(用药组),每组各6只。糖尿病组和用药组大鼠经链脲佐菌素诱导建立糖尿病模型,再制备深Ⅱ度烫伤模型,用药组大鼠建模成功后给予创面下局部皮内注射TGF-β1。烫伤后1、3、5、7、14、21 d记录创面未愈合面积并计算创面愈合率,组织学检查观察创面愈合情况,免疫组化检测创面组织TGF-β1表达。结果从烫伤后5 d起,对照组和用药组创面愈合率较糖尿病组升高,差异有统计学意义(P<0.05),对照组与用药组比较差异无统计学意义(P>0.05)。对照组创面局部TGF-β1含量较糖尿病组多,差异有统计学意义(P<0.05)。结论糖尿病大鼠烫伤创面较正常大鼠烫伤创面愈合速度慢,与创面局部TGF-β1含量减少有关。创面局部应用TGF-β1有利于糖尿病大鼠烫伤创面愈合。

Objective To observe expression of transforming growth factor β1（TGF-β1） in second-degree scald tissues in diabetic rats,and investigate the effect of TGF-β1 on the wound healing process.Methods Eighteen SD rats were randomly divided into 3 groups： the scald group（control group）,the diabetic scald group（diabetic group） and the diabetic scald with TGF-β1 treatment group（TGF-β1 group）,6 rats in each group.Rats in the diabetic group and the TGF-β1 group were treated with streptozotocin to induce diabetes,and second-degree scald models were established.After model establishment,local intra-dermal injection of TGF-β1 into the wound was performed in the TGF-β1 group.The unhealed areas of wounds were recorded and wound healing rates were calculated 1,3,5,7,14 and 21 days after scald.The healing process was evaluated by histological examination,and expression of TGF-β1 was detected by the immunohistochemiscal technique.The data were statistically analyzed.Results Five days after scald,the healing rate in the control group and the TGF-β1 group was significantly higher than that in the diabetic group（P〈0.05）,while there was no significant difference between the control group and the TGF-β1group.Compared with the control group,expression of TGF-β1 was lower in scald tissues in the diabetic group（P〈0.05）.Conclusion The unfavorable healing process is related to decreased amount of TGF-β1 in diabetic wounds.Topical application of TGF-β1 can accelerate wound healing in diabetic rats.