醒脑静注射液对蛛网膜下腔出血后海马神经元凋亡的影响

Effect of Xingnaojing on apoptosis of hippocampal neurons in rats with subarachnoid hemorrhage

目的探讨醒脑静注射液(XNJJ)对蛛网膜下腔出血(SAH)后海马相关因子凋亡的影响。方法选择成年雄性Wistar大鼠35只,随机分为空白对照组、生理盐水对照组、SAH组、XNJJ治疗1组、XNJJ治疗2组,每组7只,采用新鲜自体股动脉血枕大池二次注入法建立SAH模型,用Real-Time PCR技术检测海马神经元中凋亡分子Caspase-3和Bax以及抗凋亡分子Bcl-2在各组的变化情况,并用Western blot进一步验证此变化。结果 SAH后海马神经元中Caspase-3和Bax的表达均有不同程度上升(P<0.05),Bcl-2也有所上升(P<0.01)。XNJJ治疗1组及2组Caspase-3和Bax的表达较SAH组下降(P<0.01),而Bcl-2变化无统计学差异(P>0.05)。结论 XNJJ可以抑制海马神经元凋亡,具有脑保护作用。

Objective To investigate the effect of Xingnaojing（xnjj） on the apoptosis of hippocampal neurons in rats with subarachnoid hemorrhage（SAH）.Methods The SAH model was established by two time autologous blood injection into the cisterna magna of adult male Wistar rats.35 rats were randomly divided into 5 groups naive,saline control,SAH,xnjj treatment 1,and xnjj treatment 2 groups.Gene expressions of Caspase-3,Bax,and Bcl-2 in different groups after SAH were detected with Real-Time PCR.Further,Western blot was applied to determine protein changes of these three molecules after SAH.Results Compared with the control group,Caspase-3,Bax,and Bcl-2 increased in varying degrees in the hippocampus of the SAH group（P〈0.05）,while in Xingnaojing treatment groups,Caspase-3 and Bax,but not Bcl-2,decreased when compared with the SAH group（P〈0.01）.Conclusion Xingnaojing shows an obvious neuroprotective effect on hippocampal neurons by inhibiting SAH-related apoptosis.