黄芪注射液对同种移植小鼠糖皮质激素受体及移植物生存的影响

Effect of AMI on Glucocorticoid Receptors and graft survival in the murine allotransplantation model

目的探讨黄芪注射液(AMI)是否通过调节糖皮质激素代谢影响同种移植物生存。方法体内应用AMI后,逐日观察同种移植小鼠移植物生存状况;应用受体放射配基结合分析测定脾细胞糖皮质激素受体(GR)结合位点;利用放射免疫分析检测血清皮质醇水平。结果同种移植早期小鼠脾细胞GR结合位点数和血清皮质醇明显升高,此后逐渐下降,在排斥高峰期(第14天)达到最低值。单用AMI(60 g/kg)或环磷酰胺(CTX,100 mg/kg)及二者联合应用均可显著延长同种移植物的生存时间。AMI单独或与CTX联合应用可以明显上调排斥高峰期受体小鼠脾细胞GR位点数和血清皮质醇水平,AMI可以逆转CTX引起的GR结合位点数的下降(P<0.05)。结论 AMI可明显抑制同种移植排斥反应,与CTX联用可明显提高移植物存活率,其作用机制可能与上调GR结合位点数和血清皮质醇水平,促进内源性糖皮质激素发挥作用有关。

Objective To explore the effects of Astragalus Membranaceus Injection（AMI） on allograft survival,changes of the glucocorticoid receptor（GR） and cortisol in recipient mice.Methods AMI and CTX（Cyclophosphamide,CTX） were administered into the murine skin allotransplantation model.Allograft survival was observed daily.The amount of GR of recipient′s spleen cells was assayed by Radioligand Binding Assay and the level of serum cortisol was detected by radioimmunoassay.Results The amount of spleen GR and serum cortisol transiently increased in the early stage of transplantation and then decreased,in which the lower points appeared at the top period of the rejection（day 14）.AMI or CTX administration alone or combined in vivo obviously prolonged graft survival.AMI alone or combined with CTX up-regulated the amount of GR and serum cortisol on day 14 post transplantation（P〈0.05）.AMI combined with CTX could more significantly increase the amount of GR than CTX alone（P〈0.05）.Conclusion Administration of AMI alone or combined with CTX in vivo can significantly prolong allograft survival which may be attributed to the up-regulation of GR and serum cortisol and promotion of the endogenous glucocorticoid function.