乳腺癌组织中Id1基因mRNA的表达及其与临床病理的关系

Id1 mRNA Expression in Breast Cancer and Its Relation to Clinicopathological Features

目的观察乳腺癌组织中的分化/DNA结合抑制因子1(Id1)mRNA表达情况,并探讨其与临床病理及其他分子指标的关系。方法应用实时荧光定量逆转录——聚合酶链反应对48例乳腺癌组织,20例乳腺纤维腺瘤组织及乳腺癌MCF-7细胞系中Id1mRNA进行检测。以免疫组织化学SABC法检测Id1蛋白在乳腺癌、乳腺纤维腺瘤组织中的表达及与乳腺癌中VEGF蛋白表达的关系。结果经实时荧光定量RT-PCR法检测,乳腺癌组织,乳腺纤维腺瘤组织中的2-ΔCT值分别为:(3.4581±1.3644)、(1.1042±0.0125)。乳腺癌组织的Id1mRNA阳性表达率均显著高于乳腺纤维腺瘤组织(P<0.05),Spearman等级相关分析表明其与VEGF表达呈正相关(r=0.303,P=0.037)。而与患者的年龄,淋巴结转移,肿块大小、组织学分级,pTNM分期等临床病理因素无关(P>0.05)。结论 Id1在乳腺癌中高表达,与VEGF表达呈正相关,可能与血管形成有关。

Objective To investigate the expression of inhibitor of differentiation/DNA binding 1（Id1） in breast cancer tissue and its relation to clinicopathological and other molecular parameters.Methods The mRNA expression of Id1 in tissue and MCF-7 cell line of breast cancer were detected by fqRT-PCR.The protein expression of Id1,VEGF in 48 cases of breast carcinoma and 20 cases of breast fibroadenoma tissues of breast were detected by SABC immunohistochemistry.Results By Real-time RT-PCR method,the 2-ΔCT values of the breast carcinoma tissue and breast fibroadenoma tissue were（3.4581±1.3644） and（1.1042±0.0125） respectively.The positive rates of Id1 were significantly higher in breast carcinoma than those in breast fibroadenoma tissues,there were significant difference between the two groups（P0.05）.Spearman rank correlation analysis revealed that the protein expression of Id1 was positively correlated with that of VEGF（r=0.303,P=0.037）.No statistical correlation existed among the patients as grouped by age,lymph nodes metastasis,tumor size,histological grading,pTNM stages（P0.05）.Conclusion Over-expression of Id1 exists in the patients with breast cancer.The expression of Id1 is positively correlated with that of VEGF which may be related to blood vessel formation.