结直肠癌中人微小病毒B19衣壳蛋白的表达与COX-2的关系

Capsid Protein of Parvovirus B19 and COX-2 in Colon-rectal Adenocarcinomas

目的检测人微小病毒B19基因与COX-2表达的关系,揭示该病毒在结直肠腺癌中可能的作用机制。方法选取37例结直肠癌石蜡标本,采用免疫组织化学方法分别检测B19病毒衣壳蛋白VP1/VP2和COX-2的表达,并进行统计学分析。运用免疫组织化学双标记法检测抗原表达定位关系。采用Western blot方法检测转染pCDNA3.1/VP1u的结肠癌Lovo细胞COX-2的表达。结果衣壳蛋白和COX-2在结直肠腺癌组织中表达率的吻合度具有统计学意义。免疫组织化学双标记结果直观显示两种蛋白表达的定位关系,衣壳蛋白主要定位于细胞核内,COX-2定位细胞质内。Western blot结果示VP1u细胞转染组COX-2的表达水平明显较空载体转染组上调。结论人微小病毒B19衣壳蛋白的表达与COX-2表达具有相关性,B19病毒可能通过VP1u上调COX-2的表达从而在结直肠腺癌中发挥一定作用。

Objective To explore the relationship between the capsid protein of human parvovirus B19 and the COX-2 in colon-rectal adenocarcinoma.Methods A total of 37 paraffin-embedded colon-rectal carcinoma tissues were analyzed by IHC for the expression of capsid protein and COX-2.The location of two kinds of proteins were investigated by double-staining of IHC.The method of Western blot was used to examine the expression of COX-2 and capsid protein.Results There was statistical significance between capsid protein and COX-2 in goodness of fit.The expression of COX-2 was located in intracytoplasm whereas the capsid protein is in nucleus.Western blot showed the expression of COX-2 was up-regulated significantly in the cells transfected by VP1u compared to the controls transfected by pcDNA3.1.Conclusion These results indicate a possible role of B19 in pathogenesis of colon-rectal adenocarcinoma by VP1u up-regulating the expression of COX-2.