X线对仔鼠胃组织结构及总抗氧化能力、谷胱甘肽过氧化物酶和谷胱甘肽还原酶活性的影响

Effects of X-ray on histological structure and activities of total antioxidant capacity,glutathione peroxidase,glutathione reductase in stomach of filial mice

目的观察X线辐射后仔鼠胃组织结构和总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-PX)、谷胱甘肽还原酶(GR)的动态变化,为电离辐射防护提供实验依据。方法对160只仔鼠(出生6～7d)用不同吸收剂量(0Gy、1Gy、3Gy、5Gy、7Gy)X线进行全身辐射,分别于辐射后1d、5d、10d和20d,用比色法检测仔鼠胃组织中T-AOC、GSH-PX、GR酶活性的变化,用显微技术观察仔鼠胃组织结构的变化。结果 1Gy辐射组仔鼠胃T-AOC活性在1～20d时高于对照组,差异显著(P<0.05),GR活性在1～10d时高于对照组,差异显著(P<0.05),其他各辐射组T-AOC和GR在辐射后始终低于对照组,差异显著或极显著(P<0.05或P<0.01);1Gy辐射组仔鼠胃GSH-PX活性在辐射后1d时低于对照组,差异显著(P<0.05),以后高于对照组,差异显著(P<0.05);3Gy辐射组GSH-PX在辐射后1d时高于对照组,差异显著(P<0.05),以后始终低于对照组,差异显著或极显著(P<0.05或P<0.01);其他各辐射组GSH-PX活性始终低于对照组,差异极显著(P<0.01)。随着辐射剂量的增大,实验组仔鼠胃黏膜上皮和腺体均有不同程度的变化,上皮肿胀、空泡化、脱落,胃底腺细胞排列松散、部分降解,胃出血。结论 X线辐射影响仔鼠胃组织结构,这可能与胃抗氧化酶活性降低有关。

Objective To explore the effects of X-ray on stomach of filial mice, we investigated changes of the histological structure, activities of total antioxidant capacity （ T-AOC）, glutathione peroxidase （GSH-PX） and glutathione reductase （GR） in stomach after irradiation with different dosages of X-ray in development filial mice. Methods Totally 160 filial mice （birth 6-7 days）were irradiated with different dosages （0Gy, 1Gy, 3Gy, 5Gy, 7Gy） X-ray, and then detected the activities of T-AOC, GSH-PX, GR by colorimetry from all irradiated groups of different stages at day 1, day 5, day 10 and day 20 after irradiation. In addition, the changes of the gastric lesions of filial mice were observed by optical microscope from all experimental groups. Results The intensities of T-AOC, GR activities in stomach of the neonatal mice were lower in x-ray irradiated groups than that in the control group（ P 〈 0. 05 or P 〈 0.01 ） , with the exception for 1Gy group. The intensities of GSH-PX activities in stomach of the neonatal mice were lower in 1Gy group and higher in 3Gy group than that in the control group on the first day after irradiation （ P 〈 0. 05 ）. The activities of enzyme increased in 1 Gy group and reduced in 3Gy group at day 5-20 after irradiation, in other irradiated groups the intensities of GSH-PX were lower than that in the control group invariably （ P 〈 0. 05 or P 〈 0.01 ）. With the increase of radiation dosages, the epithelial cells of stomach mucosa and gland cell of filial mice had different degrees of change. The epithelial cells of stomach mucosa were swelling, of vacuolization and fall-off. Gastric glands were untrammeled and the stomach was hemorrhaged. Conclusion X-ray radiation affects the structure of filial mouse stomach, it might be correlated with the low activities of T-AOC, GSH-PX and GR in filial mouse stomach.