海洛因戒断与复吸对大鼠结肠5-羟色胺和P物质表达的影响

Effect of heroin withdrawal and relapse on the expressions of 5-hydroxy tryptamine and substance P in colon of rats

目的探讨海洛因戒断、脱毒和复吸期间5-羟色胺(5-HT)、P物质(SP)在大鼠结肠表达的变化。方法将正常雄性SD大鼠35只,随机分为实验组15只、盐水对照组15只和正常对照组5只,实验组大鼠又分戒断组(HWG)、脱毒治疗组(MDG)和复吸组(HRG)。取正常对照组、盐水对照组及实验组大鼠的结肠组织,采用免疫组织化学SABC法及图像分析方法进行研究。结果戒断组和复吸组大鼠结肠内的5-HT-、SP-免疫反应(IR)细胞的平均灰度值低于正常及盐水对照组,免疫反应细胞计数增多,差异有统计学意义(P<0.05)。脱毒治疗组与正常及盐水对照组比较,大鼠结肠内的5-HT-、SP-IR细胞的平均灰度值及细胞计数差异均无统计学意义(P>0.05)。结论在海洛因戒断、复吸期间,结肠分泌5-HT、SP增多;美沙酮脱毒治疗后,5-HT、SP的表达与正常组差异无显著性,提示结肠分泌的5-HT、SP参与了海洛因戒断、脱毒和复吸期间机体的调节。

Objective To investigate the changes of expression of 5-hydroxy tryptamine （5-HT）, substance P （SP） in rat colon during heroin withdrawal, methadone detoxication and heroin relapse period. Methods Thirty-five adult male SD rats were randomly divided into three groups： normal control group （NCG）, saline control group （SCG） and experimental group （EG）. Experimental group divided into： heroin withdrawal group （HWG）, methadone detoxication group （MDG） and heroin relapse group （HRG）. The colon tissues of rats in five groups were excised respectively. Immunohistochemical SABC method and image analysis were used in the research. Results Compared with NCG and SCG, the mean grey degree of 5-HT, SP immunoreactive cells decreased, the number of 5-HT, SP immunoreactive cells increased obviously in HWG and HRG（P 〈 0.05）. Compared with NCG and SCG, there were no statistical significances on the mean grey degree and cells counting of 5-HT and SP immunoreactive cells （ P 〉 0. 05 ） in MDG. Conclusion The colonic secretion of 5-HT and SP increases during heroin withdrawal and relapse. After treatment with methadone detoxication, the expression of 5-HT and SP is close to normal. The result suggests that the colonic secretion of 5-HT and SP participate in the process of body adjusting during heroin withdrawal, methadone detoxication and heroin relapse period.