外科手术联合自体骨髓干细胞动员治疗大鼠脑缺血

Surgical operation combined with autologous bone marrow stem cell mobilization to treat ischemic cerebrovascular diseases in rats

背景:研究表明干细胞治疗能够促进脑功能恢复,联合外科干预能更有效地改善预后。目的:分析外科手术联合自体骨髓干细胞动员治疗脑缺血的疗效和机制。方法:用线栓法制作SD大鼠脑缺血模型后随机分为4组:对照组不干预,其他3组分别行外科手术、重组人粒细胞集落刺激因子和粒/巨噬细胞集落刺激因子动员自体骨髓干细胞治疗或联合治疗。结果与结论:外科手术联合自体骨髓干细胞动员组大鼠的神经功能缺损评分、缺血坏死面积和细胞凋亡数明显低于对照组、外科手术组和自体骨髓干细胞动员组(P<0.01);BrdU、BDNF阳性细胞数、微血管数较其他组明显增多(P<0.05)。结果显示外科手术联合自体骨髓干细胞动员可减少大鼠脑梗死面积,抗细胞凋亡,促进神经再生和修复,改善脑功能。

BACKGROUND：Studies have shown that stem cell therapy can promote recovery of brain function,and stem cells combined with surgical intervention can be more effective in improving the prognosis. OBJECTIVE：To explore the effect and mechanism of surgical operation combined with autologous bone marrow stem cell mobilization in treatment of cerebral ischemia. METHODS：Male Sprague-Dawley rats were used to produce rat cerebral ischemia models using suture method and then divided into four groups. We used surgery,recombinant human granulocyte colony stimulating factor（rhG-CSF） and rhGM-CSF mobilization of autologous bone marrow stem cell therapy respectively. RESULTS AND CONCLUSION：In the surgery combined with autologous bone marrow stem cell mobilization group,the neurological deficit score,cerebral infarction area and the number of apoptosis were significantly lower than those in the control group,surgery group and bone marrow stem cell mobilization group（P 0.01）;Histopathology examination showed that BrdU,BDNF positive cells and angiogenesis were much more than those in the other three groups（P 0.05） . The surgery combined with autologous bone marrow stem cell mobilization can reduce the size of cerebral infarction,inhibit cell apoptosis,promote nerve regeneration and repair,and then improve brain function.