远志总皂苷干预β-淀粉样蛋白致伤神经干细胞Caspase-3及Par-4的表达

Effect of tenuigenin on the Caspase-3 and Par-4 expression of neural stem cells induced by beta-amyloid protein

背景:远志总皂苷具有良好的神经保护作用。目的:分析远志总皂苷对β-淀粉样蛋白致伤海马神经干细胞的保护作用及机制。方法:自昆明小鼠海马分离培养神经干细胞,取第3代神经干细胞,用含不同质量浓度远志总皂苷与β-淀粉样蛋白致伤体外培养的神经干细胞共孵育。结果:应用免疫组织化学法检测Caspase-3阳性神经干细胞,与对照组相比,远志总皂苷组Caspase-3阳性细胞率及Par-4的表达明显降低,差异具有显著性意义(P<0.05)。提示远志总皂苷能够降低β-淀粉样蛋白致伤神经干细胞中Caspase-3及Par-4的表达。

BACKGROUND：The effect of tenuigenin which has a good nerve protection to neural stem cells has not been reported. OBJECTIVE：To investigate the protective effect and mechanism of tenuigenin for neural stem cells in the hippocampus impaired by β-amyloid protein. METHODS：The third passage neural stem cells generated from the hippocampi of Kunming mice which injured by β-amyloid protein in vitro were incubated with different concentrations of tenuigenin. RESULTS AND CONCLUSION：Immunocytochemical technique was used to detect Caspase3-positive neural stem cells. The study revealed that the expression of Par-4 and caspase-3 positive neural stem cells in the tenuigenin group were significantly lower than that in the control group with statistical significance（P 0.05） . Tenuigenin can reduce the expression of Par-4 and Caspase-3 in neural stem cells impaired by β-amyloid protein.