摘要
背景:远志总皂苷具有良好的神经保护作用。目的:分析远志总皂苷对β-淀粉样蛋白致伤海马神经干细胞的保护作用及机制。方法:自昆明小鼠海马分离培养神经干细胞,取第3代神经干细胞,用含不同质量浓度远志总皂苷与β-淀粉样蛋白致伤体外培养的神经干细胞共孵育。结果:应用免疫组织化学法检测Caspase-3阳性神经干细胞,与对照组相比,远志总皂苷组Caspase-3阳性细胞率及Par-4的表达明显降低,差异具有显著性意义(P<0.05)。提示远志总皂苷能够降低β-淀粉样蛋白致伤神经干细胞中Caspase-3及Par-4的表达。
BACKGROUND:The effect of tenuigenin which has a good nerve protection to neural stem cells has not been reported. OBJECTIVE:To investigate the protective effect and mechanism of tenuigenin for neural stem cells in the hippocampus impaired by β-amyloid protein. METHODS:The third passage neural stem cells generated from the hippocampi of Kunming mice which injured by β-amyloid protein in vitro were incubated with different concentrations of tenuigenin. RESULTS AND CONCLUSION:Immunocytochemical technique was used to detect Caspase3-positive neural stem cells. The study revealed that the expression of Par-4 and caspase-3 positive neural stem cells in the tenuigenin group were significantly lower than that in the control group with statistical significance(P 0.05) . Tenuigenin can reduce the expression of Par-4 and Caspase-3 in neural stem cells impaired by β-amyloid protein.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第23期4282-4286,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金资助(30971039):<Mash1通路在NGF诱导神经干细胞向胆碱能神经元定向分化中的调控作用及机制>
黑龙江省自然科学基金资助(D2007-02):<Noggin基因修饰神经干细胞移植治疗局灶性脑缺血的实验研究>
黑龙江省自然科学基金资助(D200617):神经干细胞移植治疗缺血性脑血管病及移植环境优化的实验研究~~