四种中药煎剂和雪莲花醇提液对骨髓间充质干细胞增殖活性的影响

Effect of four traditional Chinese medicine decoctions and ethanolic extract from Saussurea involucrata on bone marrow mesenchymal stem cell proliferation activity in vitro

背景:中药可能具有促进骨髓间充质干细胞增殖的作用。目的:观察单味中药黄芪、丹参、仙灵脾和复方右归饮煎剂及雪莲花醇提液对大鼠骨髓间充质干细胞体外培养增殖活性的影响。方法:雪莲花乙醇浸泡后定容,其他单味和复方中药水煎定容,高压灭菌。取大鼠股骨和肱骨,分离骨髓间充质干细胞,常规培养传代3次后,加入煎剂及雪莲花醇提液。其浓度分为10-2,10-3,10-4及10-5,作用3d后用四甲基偶氮唑盐法测定细胞活力。结果与结论:黄芪煎剂和右归饮煎剂及雪莲花醇提液4个浓度组均可促进骨髓间充质干细胞增殖(P<0.05)。提示雪莲花醇提液、右归饮煎剂和黄芪煎剂均具有促进大鼠骨髓间充质干细胞体外培养增殖活性的作用。

BACKGROUND：Recent medical literature reports indicate Chinese medicine can promote bone mesenchymal stem cells（BMSCs） proliferation.OBJECTIVE：To explore the effect of single herb Radix Astragali,Salvia,Herba Epimedii,and compound Youguiyin decoction and ethanolic extract of Saussurea involucrata on rat BMSCs proliferative activity in vitro.METHODS：BMSCs were isolated and cultured from rat femur and humerus by conventional method.Through high pressure sterilization,the ethanolic extract of Saussurea involucrata and other single herb and compound herb decoction were administered to the third passage BMSCs with 10-2,10-3,10-4,10-5 concentrations.The cell proliferative activity was determined by MTT method 3 days after culture.RESULTS AND CONCLUSION：The herb decoction of Radix Astragali,Youguiyin and the ethanolic extract of Saussurea involucrata promoted BMSCs proliferation with all four concentrations（P 0.01） .The ethanolic extract of Saussurea involucrata and the herb decoction of Radix Astragali and Youguiyin showed the positive effect on rat BMSCs proliferative activity in vitro.