川芎嗪联合创伤性脑组织匀浆液诱导骨髓间充质干细胞向神经样细胞分化

Ligustrazine and traumatic brain tissue homogenate for inducing bone marrow mesenchymal stem cells differentiate into neuron-like cells

背景:川芎嗪和创伤性脑组织匀浆液均可诱导骨髓间充质干细胞向神经样细胞分化。目的:探讨川芎嗪与创伤性脑组织匀浆液诱导骨髓间充质干细胞向神经样细胞分化的联合效应。方法:分离培养Wistar大鼠骨髓间充质干细胞后分为4组,加入不同的诱导培养基分别干预:空白对照组、川芎嗪诱导组、创伤性脑匀浆液诱导组和川芎嗪联合创伤性脑匀浆液诱导组。诱导后采用倒差显微镜观察细胞形态变化,并统计不同时段四组细胞分化率。分别取部分细胞进行神经元特异性烯醇化酶染色,胶质纤维酸性蛋白免疫细胞化学与免疫荧光细胞化学双标检测。结果与结论:川芎嗪及受损大鼠脑匀浆液上清液可诱导大鼠骨髓间充质干细胞向神经元样细胞分化,随诱导时间的延长,诱导分化率越高,具有较重要的应用价值,而神经元特异性烯醇化酶与胶质纤维酸性蛋白在其分化信号中起重要作用。

BACKGROUND：Both ligustrazine and traumatic brain tissue homogenate could induce bone marrow mesenchymal stem cells（BMSCs） differentiate to neuron-like cells.OBJECTIVE：To investigate the combined effects of ligustrazine and traumatic brain tissue homogenate for inducing BMSCs differentiate into neuron-like cells.METHODS：BMSCs isolated from Wistar rats were randomly divided into four groups：blank control,ligustrazine induction,traumatic brain homogenate induction,and combined induction.The morphology of the cells were observed under inverted microscope,and the differentiation rate of cells at different time points was statistically analyzed.Some cells were determined with neuronspecific enolase,glial fibrillary acidic protein immunocytochemistry and immunofluorescence staining.RESULTS AND CONCLUSION：The ligustrazine combined with traumatic brain tissue homogenate can induce BMSCs differentiate into neuron-like cells,the induced differentiation rate increased as the inducting time,so the combination has an important application value.Neuronspecific enolase and glial fibrillary acidic protein are of great significance in the differentiation signals.