粉防己碱对急性缺血性肾损伤细胞凋亡的影响

Changes of apoptosis in rats of acute ischemic renal injury under the treatment of tetrandrine

目的 :探讨粉防己碱在急性缺血性肾损伤中的作用及与细胞凋亡的关系。方法 :在钳夹双侧肾蒂 45min致大鼠急性缺血性肾损伤模型的基础上 ,采用肾组织切片HE染色、肾组织DNA片段分析及原位末端转移酶标记法等技术 ,定性、定量分析了急性缺血性肾损伤时肾小管上皮细胞的凋亡现象。结果 :急性缺血性肾损伤时 ,肾小管上皮细胞的凋亡现象明显增加 ;粉防己碱对急性缺血性肾损伤具有保护作用 ,能够明显降低受损肾小管的细胞凋亡水平。结论 :急性缺血性肾损伤时 ,中药粉防己碱能够调节肾小管上皮细胞的凋亡水平 。

Aim:To elucidate the effect of tetrandrine on acute ischemic renal injury and the relation between tetrandrine and apoptosis.Methods:A model for bilateral post-ischemic renal injury in rats was developed by calmping renal pedicles for 45 minutes,renal tissular DNA fragmentation analysis and renal tissular HE staining were used.Also the quantative analysis of apoptosis in injuried renal tubular epithelial was carried out by using TdT mediated dUTP nick and labeling (TUNEL).Results:Apoptosis of renal tubular epithelial increased in acute ischemic renal injury.Tetrandrine can remarkably decrease the level of apoptosis in injuried renal tubule while protecting renal tissue against the ischemic injuries.Conclusion:Tetrandrine can adjust the level of apoptosis in renal tubular epithelial and alleviate renal tissular injury.