补阳还五汤提高血管性痴呆大鼠海马CREB、C/EBP的DNA结合活性

Effect of Buyanghuanwutang on the improvement of the DNA-binding activity of CREB and C/EBP in hippocampus of vascular dementia rats

目的观察补阳还五汤对血管性痴呆大鼠海马CREB(cAMP反应元件结合蛋白)、C/EBP(CCAAT/增强子结合蛋白)DNA结合活性的影响。方法实验分为假手术组、模型组、补阳还五汤组和尼莫地平组。采用改良的4血管法制备血管性痴呆模型,分别给予生理盐水、补阳还五汤、尼莫地平灌胃干预,用凝胶电泳迁移率改变分析法(EMSA)测定各组大鼠CREB和C/EBP的DNA结合活性。结果与假手术组比较,模型组大鼠海马组织CREB的DNA结合活性明显降低(P<0.01);与模型组比较,尼莫地平组与补阳还五汤组明显升高(P<0.01);尼莫地平组与补阳还五汤组之间差异无显著性(P>0.05)。结论补阳还五汤可提高VD大鼠海马组织CREB、C/EBP与DNA的结合活性。

Aim To observe the influence of Buyanghuanwutang（BYHWT） on the DNA-binding activity of cAMP response element binding protein（CREB） and CCAAT/enhancer binding protein（C/EBP） in hippocampus of vascular dementia（VD） rats.Methods The rats were randomly divided into sham-operation group,VD model group,BYHWT-treated group and nimodipine-treated group.VD rat models were prepared by four-vessel occlusion,and physiological saline solution,BYHWT and nimodipine were administered respectively to four groups by gavage.Electrophoretic Mobility Shift Assay（EMSA） was used to observe the change of DNA-binding activity of CREB and C/EBP.Results The DNA-binding activity of CREB of rat hippocampus was significantly lower in VD model group than that in Sham-operation group（P0.01）.Compared with VD model group,both nimodipine-treated group and BYHWT-treated group were definitely higher（P0.01）.There was no significant difference between BYHWT-treated group and nimodipine-treated group（P0.05）.Conclusion BYHWT can improve the DNA-binding activity of CREB and C/EBP in hippocampus of VD rat.