NaGdF_4:Yb,Er纳米颗粒的合成及对HeLa细胞的生物标记

Synthesis of NaGdF_4:Yb,Er Nanoparticles and Their Application in Cell Biolabeling

以稀土硬脂酸盐为前驱体,采用溶剂热法合成了单分散的NaGdF4:Yb,Er上转换荧光纳米颗粒.通过透射电子显微镜(TEM)、X射线衍射仪(XRD)、荧光(FL)光谱仪、磁滞回线(MHL)等对合成的样品进行了表征.所合成的纳米颗粒粒径均一,平均粒径为20 nm左右,形状规则,结晶度高,发光性能良好,同时具有优越的顺磁性.利用经典的Stober法对纳米颗粒进行了表面氨基修饰,将修饰后的纳米颗粒与转铁蛋白结合制成探针,实现了对HeLa细胞的标记与成像.标记结果表明:该方法特异性较好,且红外光激发可有效避免来自生物样品的自体荧光干扰.

The monodispersed NaGdF4：Yb,Er nanoparticles were synthesized via a solvothermal approach.Rare-earth stearate was used as the precursor.The properties of as-prepared nanoparticles were characterized by transmission electron microscopy （TEM）,X-ray diffraction（XRD）,fluorescence spectra and magnetic hysteresis loop（MHL）.Experimental results showed that the nanoparticles are single crystal hexagonal phases with an average size of around 20 nm,exhibiting good superparamagnetic and excellent up-conversion fluorescent characteristics.The as-prepared nanoparticles were modified with amino groups by Stober method,and linked with transferrin to make it to be a probe.The probe was applied to the bio-labeling and imaging of HeLa cells.This method was proved to have good specificity,and the infrared light excitation can effectively avoid the autofluorescence of biological samples.