虫草菌丝抗肝纤维化的实验研究

Effects of Cordyceps Sinensis on CCl_4-induced Hepatic Fibrosis in Rats

目的:观察人工虫草菌丝对CCl_4中毒性肝纤维化的预防作用。方法:复制大鼠CCl_4中毒性肝纤维化模型,并以秋水仙碱作为阳性治疗对照,采用光镜、电镜观察肝脏组织学改变,测定血清ALT、Alb、G、HA、LN、肝组织Hyp含量。结果:虫草菌丝组肝细胞损害、肝脏脂肪变性、炎性细胞浸润的程度均较模型组轻,但再生肝细胞较多,有假小叶形成;其对肝功能、肝纤维化指标也有明显改善,但秋水仙碱的抗肝纤维化作用优于虫草菌丝。结论:虫草菌丝对肝脏CCl_4损伤具有一定保护作用,有较强促进肝细胞再生修复作用,同时在损伤肝组织中成纤维细胞及胶原纤维也明显增生,其与活血化瘀药有机伍用,可能是防治肝纤维化较理想的组合。

Aim: To investigate the effects of Cordyceps Sinensis (CS) on CCl4-induced hepatic fibrosis (HF). Methods: The model of CCl4 induced HF in rats was treated with CS or Colchicine (Col). The histological changes were observed with light and electronic microscopes. The serum concentrations of alanine aminotransferase (ALT). albumin, globulin, hyaluronic acid (HA), Laminin (LN) were determinde and hydroxyproline (Hyp) in liver tissue were also measured. Results: The extent of hepatocytic lesion, fatty degeneration and infiltration of inflammatory cells was lighter in the CS group than in the model group. CS had stronger effect on improving regeneration of hepatocytes and proliferation of fibroblasts and collagenous fibers. Conclusion: CS can exert significant effects on improving regeneration and repair of hepatocytes. Meanwhile, proliferation of fibroblasts and collagenous fibers were also obviously observed.