利用延迟荧光光谱F_(730)/F_(685)的逆境胁迫生理检测方法

Detection of Stress Physiology of Plant Using F_(730)/F_(685) Ratio of Delayed Fluorescence Spectrum

以玉米98-2为样品,实验分析了高温、盐及紫外UV-B胁迫下,样品离体叶片延迟荧光发射光谱的变化。标记685 nm主峰带的最高峰值为F685,730 nm次峰带的最高峰值为F730,实验发现不同的逆境胁迫对比值F730/F685均产生了显著的影响。结合样品在相同逆境下叶绿素荧光参数Fv/Fm以及DF强度的检测实验,结果表明,无论是在哪种逆境胁迫下,无论是对胁迫程度还是胁迫时间的响应特性上,F730/F685、DF强度与Fv/Fm均有着较好的一致性,线性拟合相似度大于0.960 9。因此,通过对本文实验结果的分析认为,延迟荧光光谱峰值比F730/F685的逆境胁迫响应与叶片受胁迫影响的程度密切相关,且可能是一种检测植物逆境光合生理的新方法。

Delayed fluorescece（DF） is intrinsically related to the photosynthesis process,and it is a good method of researching the effect of various chemical and physical factors in vivo and in vitro on photosynthetic performance of plants.In this paper,the detection of DF emission spectra under the stress（heat,salt and UV-B） have been investigated in maize（98-2） sample.The maximal values of DF emission spectra at 685 nm and 730 nm were marked as F685 and F730 respectively.After comparative measurement of F730/F685 ratio,DF intensity and Fv/Fm,the linear fitting results shown that the two parameters has a good linear correlation（R2≥0.960 9）.The experimental results suggest that the degree of plant photosynthetic physiology damage whether caused by different concentration or different stress time can be effectively measured by F730/F685.Moreover,the responses of F730/F685 ratio of DF emission spectra to the stress could be a new and useful judgment to identify and evaluate the stress damage of the samples.