玉米秸秆青贮添加剂的研究与应用

A study and application on silage additives for corn stalk

筛选得到高产乳酸菌株WGY-3,利用正交试验法确定出WGY-3菌株液体发酵的培养基配方为:葡萄糖20g/L、豆粕25g/L、西红柿汁20g/L、K2HPO41g/L、Na2HPO42g/L。最佳液体发酵条件:pH值6～7,温度为38℃,接种量为4%,发酵时间15h,WGY-3菌株的最佳添加量为188ml/(100g玉米秸秆)。

The high-yield lactobacillus strain WGY-3 was screened,and the cultivation medium formula for WGY-3 strain liquid fermentation determined by the orthogonal experimental method：dextrose 20 g/L,soybean meal 25 g/L,tomato juice 20 g/L,K2HPO4 1 g/L and Na2HPO4 2 g/L.Optimum liquid fermentation conditions：pH 6~7,temperature 38℃,inoculum size 4%,fermentation time 15 h,and optimal addition of WGY-3 strain 188 ml/（100 g corn stalk）.