Twist和E-cadherin在非小细胞肺癌中的表达及意义

The study on significance of Twist and E-cadherin proteins expression in non-small-cell lung cancer

目的探讨Twist、E-cadherin蛋白在非小细胞肺癌(NSCLC)的表达及其临床意义。方法应用免疫组织化学方法检测54例NSCLC肺癌标本组织、10例正常肺组织和10例肺良性病变中Twist、E-cadherin的表达。结果 Twist在NSCLC癌组织中的表达(68.5%)明显高于正常肺组织(10%)和肺良性病变组织(20%)(P<0.05);E-cadherin表达率40.7%(22/54),明显低于正常肺组织(100%)和肺良性病变(100%)(P<0.05);Twist、E-cadherin在NSCLC癌组织中表达与肿瘤的分化程度、临床分期及淋巴结转移有关;Twist的表达与E-cadherin的表达存在明显负相关(P<0.05)。结论 Twist蛋白可能通过下调E-cadherin的表达,在NSCLC的浸润、转移过程中发挥重要作用,二者的检测对肺癌的诊断及治疗具有指导意义。

Objective To investigate the expression and clinical significance of Twist and E-cadherin in non-small-cell lung cancer（NSCLC） tissues.Methods The expression of Twist and E-cadherin in 54 cases of NSCLC tissues,10 cases of normal lung tissues and10 of cases benign lesion of lung was detected by using immunohistochemistry.Results 68.5%,10% and 20%,respectively.The positive rates of Twist in NSCLC（68.52%） was higher than that in normal lung tissues（10%） and benign lesion of lung（20%）（P0.05）.The positive rates of E-cadherin in NSCLC（40.7%） was lower than that in normal lung tissues（100%） and benign lesion of lung（100%）（P0.05）.The difference of Twist expression was significant in group of tumor differentiation,clinical stage and lymphatic metastasis.P0.05）.Negative correlation was obtained between the expression of Twist and E-cadherin proteins（r=-0.68,P=0.000）.Conclusion Twist protein in NSCLC might play an important role in the infiltration and metastasis of the tumor by down-regulating the expression of E-cadherin.The test of Twist and E-cadherin expressions may be useful for diagnosis and therapy of lung cancer.