人工活性骨内部微细胞构建的实验研究

Study of internal micro cells building method of artificial active bone

目的探讨利用快速成型技术(RP)制作特定外形的多孔β磷酸三钙(β-TCP)构建人工活性骨进行骨缺损修复的可行性。方法将24只新西兰大白兔制作右侧桡骨标准骨缺损不愈合模型,采用RP技术SL法制作具与兔桡骨缺损外形一致的多孔β-TCP支架,并该组大白兔分为2组,A组通过多孔β-TCP支架+真空冻干吸附技术复合BMP,构建人工活性骨;B组单纯采用多孔β-TCP支架,然后在槽形支架中植入自体松质骨,术后2、4、8、12周行影像学、组织学、成骨量、生物力学和骨密度的观测。结果两组材料在植入后12周后,均出现牢固的骨连接,但A组在成骨量、骨密度及生物力学指标方面优于B组(P<0.05)。结论利用RP技术进行人工活性骨的构建是可行,多孔β-TCP复合支架有利于提高体内新骨生成,达到临床修复的目的 。

Objective To explore the feasibility of the porous beta specific appearance tris calcium（beta TCP） by using rapid prototyping（RP） production technology building artificial active bone to repair defect bones.Methods Twenty-four New Zealand big white rabbits were made as the model of right radial standard bone defect nonunion,adopting RP technology SL legal system with radial defect for uniform appearance with rabbit of porous beta TCP stents,and these rabbits were divided into two groups： A group of through porous beta TCP stents ＋ vacuum freeze-drying adsorption technology,building artificial active compound BMP bone;B group using porous beta TCP stents,then in groove in the bracket auto-grafting cancellous bone.And then to observe the image,histology,bone mass and biomechanics at 2,4,8,and 12 weeks of postoperative.Results Two groups of materials at 12 weeks after implantation,all appeared solid bone connection,but bone mass,bone mineral density and the biomechanics index in A group significantly better than that in group B,P0.05.Conclusion Using RP technology to the construction of artificial active bone is feasible,porous beta TCP composite stents to increase new bone formation,and to get the purpose of clinical repair.