无机碳源对栅藻LX1生长及油脂积累特性的影响

Effect of Inorganic Carbon Supplement on the Growth and Lipid Accumulation Properties of Scenedesmus sp.LX1

以二级出水为培养基,比较了对照组和补充碳源(Na2CO3、NaHCO3和CO2)组栅藻LX1的生长和油脂积累特性.培养14d,4组试验中栅藻LX1的生长及油脂积累特性均无显著差别,藻细胞生物量、油脂及三酰甘油酯产量分别为0.70～0.77、0.27～0.34和0.029～0.033 g.L-1,单位藻细胞油脂含量约为35%～45%.藻细胞光合作用导致水体pH升高至9.2左右,有利于CO2向水中传质.对照组中,传质进入二级出水的CO2总量为1.0 g.L-1,占藻细胞固定CO2总量的97%.计算结果表明,通过传质进入二级出水中的CO2能够满足栅藻LX1生长及油脂合成对碳的需求.因此,利用二级出水培养栅藻LX1,无需额外添加碳源.

With secondary effluent as growth medium,the growth and lipid accumulation properties of Scenedesmus sp.LX1 in the control and carbon supplement（Na2CO3,NaHCO3 and CO2） groups were compared.After 14 days of cultivation,there was no significant difference in growth and lipid accumulation of Scenedesmus sp.LX1 among the 4 groups.The algal biomass,lipid and triacylglycerols production were 0.70-0.77 g·L-1,0.27-0.34 g·L-1 and 0.029-0.033 g·L-1,respectively,and the lipid content per algal biomass was 35%-45%.The microalgal photosynthesis would increase the water＇s pH to about 9.2,which could induce CO2 transferring into secondary effluent.In control group,the amount of CO2 transferring from atmosphere into secondary effluent was 1.0 g·L-1,occupying 97% of total fixed CO2 by microalga.Calculation shows that,the CO2 transferring from atmosphere into secondary effluent is enough to support microalgal growth and lipid accumulation.Therefore,to cultivate Scenedesmus sp.LX1 in secondary effluent,there is no need of carbon supplement.