表面活性剂促进剩余污泥酶水解的研究

Enhanced Enzymatic Hydrolysis of Excess Sludge by Surfactant

为了提高污泥酶水解的效率,研究向污泥中投加表面活性剂十二烷基硫酸钠(sodium dodecyl sulfate,SDS)强化污泥酶水解的效果.结果表明,SDS极大促进了剩余污泥酶水解,且复合酶的处理效果优于单酶.复合酶的投加量为0.06 g/g时,SCOD溶出率随SDS投加量成正比增加.SDS为0.20 g/g时,SCOD/TCOD从初始污泥的1.3%上升到54.3%,同时VSS去除率显著提高,最高可达43.2%.SDS的加入提高了酶的活性,当SDS为0.10 g/g时,蛋白酶活和淀粉酶活分别是单独加酶组的2.3倍和1.2倍.水解过程的前4 h,SDS+复合酶组的蛋白质、氨氮和可溶性糖浓度相应地分别提高了85.4%、92.5%和64.0%.此外,污泥水解过程的前4 h符合一级反应动力学,速率常数(K)值从空白组的0.23增加到0.41,说明SDS+复合酶的加入使得反应速率明显得到了提高.

In order to enhance the efficiency of enzymatic hydrolysis of excess sludge,sodium dodecyl sulfate（SDS） was added to the system to explore the feasibility of promotion the enzyme hydrolysis.The results showed that the enzymatic hydrolysis of excess sludge could be greatly improved by SDS,and the mixed enzymes system was more effective than that by single enzyme system.SCOD releasing increased linearly with the increase of SDS dosage at the mixed enzymes concentration of 0.06 g/g.SCOD/TCOD increased from 1.3% to 54.3% and VSS reduction achieved to 43.2% at the SDS dosage of 0.20 g/g.Further studies indicated that SDS could improve the activity of external enzymes.At SDS dosage of 0.10 g/g,the protease activity of SDS＋protease showed a 2.3-time increase and the amylase activity of SDS＋amylase showed a 1.2-time increase compared with enzymatic treatment.After 4 h hydrolysis,the concentration of protein,NH＋4-N and soluble sugar in SDS＋mixed enzymes system were improved by 85.4%,92.5% and 64.0%,respectively.Correspondingly,sludge hydrolysis within prior 4 h was consistent with first-order reaction dynamics.The reaction rate constant（K） of soluble sugar increased from 0.23 to 0.41,which indicated that the reaction rate of hydrolysis increased significantly.