摘要
研究好氧反硝化菌的筛选、生物脱氮机制及代谢特征.采用极限稀释及平板划线法对好氧条件下能同步硝化/反硝化的细菌进行广泛筛选,分离到6株具有好氧脱氮效果的异养菌,其中YY-5菌具有高效的好氧脱氮能力.对该菌株好氧脱氮过程气相和液相中可能生成的气态产物以及硝态氮等形式的含氮产物变化进行定量检测分析,探讨脱氮过程氮素的去向及其脱氮途径.YY-5菌好氧条件下脱氮过程中总氮的去除与氮气生成量之间基本保持平衡,并且在脱氮过程中没有检测到N2O气体生成,液相中也没有出现硝酸盐和亚硝酸盐的积累;YY-5菌羟胺氧化酶提取液具有较高活性,其羟胺降解率可达到70.9%.采用16S rRNA同源性比对法对分离到的细菌进行分析,结果显示与不动杆菌属(Acinetobacter sp.)同源性较高.
To screen aerobic heterotrophic denitrifying bacteria from various samples and detect metabolites quantitatively during their denitrification process.The bacteria were screened by limit dilution and crossed separation.Nitrate and nitrite were determined using phenol disulfonic acid and N-（1-naphthyl）-ethylene diamine by ultraviolet spectrophotometry.The N2 was detected by gas chromatography.Six aerobic heterotrophic bacteria were isolated and YY-5 was demonstrated to be the greatest denitrifer.The degradation rate of its hydroxylamine oxidoreductase can reached to 70.9%.Above all,there is a good balance between the removal of total nitrogen and production of N2 in process.No N2O was produced and no nitrate or nitrite were accumulated.The high ability of aerobic heterotrophic bacterium YY-5 for denitrification was screened,which showed higher homology with Acinetobacter genus through 16S rRNA blast.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2011年第8期2409-2413,共5页
Environmental Science
基金
国家高技术研究发展计划(863)项目(2009AA06Z312)
关键词
脱氮
异养菌
筛选
气态产物
羟胺氧化酶
denitrification
heterotrophic bacteria
screening
gaseous products
hydroxylamine oxidoreductase
