氟化物的细胞转化作用研究

THE STUDY ON THE CELL TRANSFORMATION OF FLUORIDE

应用金黄地鼠胚胎细胞转化试验检测了氟化物的诱变致癌性。结果显示，１、４ｍ ｍ ｏｌ／Ｌ氟化钠（ＮａＦ）受试组与阴性对照存活约８０ｄ，未见转化发生。而２ｍ ｍ ｏｌ／ＬＮａＦ处理组（ＳＨＥＦ２）细胞逐渐出现类似阳性对照ＭＮＮＧ 诱导的转化细胞特征；培养至１１０ｄ 有转化集落形成；接种培养１５０ｄ 的ＳＨＥＦ２ 于裸鼠腹部皮下有纤维肉瘤形成。

The Syrian hamster embryo cultured cells were exposed to sodium fluoride(NaF)for 24 hours with concentrations of 1mmol/L,2mmol/L and 4mmol/L for cell transformation.A dose depended cytotoxity was observed.Moreover,the groups of 1mmol/L,4mmol/L and controlled were not transformed,which had a limited lifetime of about 80 days.Mass culture treated with 2mmol/L NaF(SHEF2)escaped senescence and gradually showed characters of transformed cell that is similar to those of positive controlled. SHEF2 cells proliferated rapidly with active function,and formed transformed clonies when cultured to 110 days.Incubation with agglutinin resulted in strong agglutination,furthermore,fibrosarcoma developed in nude mice inoculated with SHEF2 cells.All the phenomena suggested that malignant transformation of SHEF2 cells had occurred.