七氟醚麻醉对新生大鼠杏仁核DNA甲基转移酶mRNA表达的影响

Effect of sevoflurane anesthesia on DNA methyltransferase mRNA expression in neonatal rat amygdala

目的探讨七氟醚麻醉对新生大鼠杏仁核DNA甲基转移酶（DNMT）mRNA表达的影响。方法新生（出生8d）SD大鼠42只，采用随机数字表法，将其随机分为2组（n=21），对照组：不给予任何处理；实验组：吸入5％七氟醚1min后，吸入浓度调整为3％维持4h。实验组分别于停止吸入即刻及停止吸入后24h时，处死大鼠，取杏仁核，测定DNMT，mRNA、DNMT3。mRNA、DNMT3。mRNA的表达；停止吸入即刻取动脉血样，进行血气分析。对照组于相应时间点进行处理。结果与对照组比较，实验组DNMTjmRNA、DNM％。mRNA表达均下调（P〈0．05），DNMT3bmRNA表达和血气分析指标差异无统计学意义（P〉0．05）。结论七氟醚麻醉可下调新生大鼠杏仁核DNM％mRNA和DNM％。mRNA的表达，该作用可能会导致发育期中枢神经功能损伤。

Objective To investigate the effect of sevoflurane anesthesia on DNA methyltransferase （DNMT） mRNA expression in neonatal rat amygdala. Methods Forty-two 8-day-old SD rats were randomly divided into 2 groups （n = 21 each）： control group and experimental group. 5% sevoflurane was inhaled for 1 rain, and then the inhaled concentration of sevoflurane was decreased to 3% and maintained for 4 h. The rats were sacrificed at the end of sevoflurane inhalation and 24 h after the end of sevoflurane inhalation, and amygdala was removed for determination of DNMT1 mRNA, DNMT3a, mRNA and DNMT3b mRNA expression by RT-PCR. Blood samples were taken at the end of sevoflurane inhalation for blood gas analysis. Results Compared with control group, the DNMT1 mRNA and DNMT3, mRNA expression was down-regulated （P 〈 0.05 ）. There were no significant differences in DNMT3b mRNA expression and parameters of blood gas analysis between the two groups （P 〉 0.05 ）. Conclusion Sevoflurane anesthesia can down-regulate DNMT1 mRNA and DNMT3. mRNA expression in neonatal rat amygdala, which may result in functional deficits during the development of central nervous system.